Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 14 mM, activation of isoenzyme F3GT1 to 160%, activation of isoenzyme F3GT2 to 136% | Euonymus alatus | |
EDTA | slight activation | Euonymus alatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 10 mM, 9% inhibition | Euonymus alatus | |
CoCl2 | 1 mM, 50% inhibition of isoenzyme F3GT1 and 25% inhibition of isoenzyme F3GT2 | Euonymus alatus | |
Cu2+ | 1 mM CuCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2 | Euonymus alatus | |
dithioerythritol | - |
Euonymus alatus | |
EDTA | 10 mM, slight inhibition | Euonymus alatus | |
iodoacetamide | - |
Euonymus alatus | |
iodoacetate | - |
Euonymus alatus | |
KCl | 10 mM, 9-10% inhibition | Euonymus alatus | |
Mn2+ | 10 mM MnCl2, 52% inhibition of isoenzyme F3GT1 and 28% inhibition of isoenzyme F3GT2 | Euonymus alatus | |
NEM | - |
Euonymus alatus | |
PCMB | - |
Euonymus alatus | |
Phenylmercuriacetate | - |
Euonymus alatus | |
Zn2+ | 1 mM ZnCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2 | Euonymus alatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM, slight stimulatory effect on isoenzyme F3GT1 and F3GT2 | Euonymus alatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Euonymus alatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euonymus alatus | - |
f. Ciliato-dentatus | - |
Purification (Comment) | Organism |
---|---|
isoenzyme F3GT1 and F3GT2 | Euonymus alatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Euonymus alatus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + isorhamnetin | - |
Euonymus alatus | UDP + isorhamnetin 3-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + kaempferol 7-O-glucoside | isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 5% of the activity with kaempferol | Euonymus alatus | UDP + kaempferol 3,7-O-diglucoside | - |
? | |
UDP-alpha-D-glucose + quercetin 7-O-beta-D-glucoside | isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 6% of the activity with kaempferol | Euonymus alatus | UDP + quercetin 3,7-O-di-beta-D-glucoside | - |
? | |
UDPglucose + 3,5,7-trihydroxyflavanone | isoenzyme F3GT1: 22% of the activity with kaempferol, isoenzyme F3GT2: 12% of the activity with kaempferol | Euonymus alatus | UDP + 3,5,7-trihydroxyflavanone 3-O-glucoside | - |
? | |
UDPglucose + 7,4'-dihydroxyflavonol | isoenzyme F3GT1: 33% of the activity with kaempferol, isoenzyme F3GT2: 34% of the activity with kaempferol | Euonymus alatus | UDP + 7,4'-dihydroxyflavonol 3-O-glucoside | - |
? | |
UDPglucose + dihydrokaempferol | isoenzyme F3GT1: 7% of the activity with kaempferol, isoenzyme F3GT2: 7% of the activity with kaempferol | Euonymus alatus | UDP + dihydrokaempferol 3-O-glucoside | - |
? | |
UDPglucose + dihydroquercetin | isoenzyme F3GT1: 3% of the activity with kaempferol, isoenzyme F3GT2: 2% of the activity with kaempferol | Euonymus alatus | UDP + dihydroquercetin 3-O-glucoside | - |
? | |
UDPglucose + fisetin | - |
Euonymus alatus | UDP + fisetin 3-O-glucoside | - |
? | |
UDPglucose + kaempferol | - |
Euonymus alatus | UDP + kaempferol 3-O-glucoside | - |
? | |
UDPglucose + kaempferol 5,7,4'-trimethyl ether | isoenzyme F3GT1: 52% of the activity with kaempferol, isoenzyme F3GT2: 11% of the activity with kaempferol | Euonymus alatus | UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside | - |
? | |
UDPglucose + kaempferol-4'-O-methylether | - |
Euonymus alatus | UDP + ? | - |
? | |
UDPglucose + myricetin | - |
Euonymus alatus | UDP + myrecetin 3-O-glucoside | - |
? | |
UDPglucose + quercetin | - |
Euonymus alatus | UDP + quercetin 3-O-glucoside | - |
? | |
UDPglucose + quercetin 5-methyl ether | isoenzyme F3GT1: 38% of the activity with kaempferol, isoenzyme F3GT2: 25% of the activity with kaempferol | Euonymus alatus | UDP + quercetin 5-methyl ether 3-O-glucoside | - |
? | |
UDPglucose + rhamnetin | isoenzyme F3GT1: 20% of the activity with kaempferol, isoenzyme F3GT2: 82% of the activity with kaempferol | Euonymus alatus | UDP + rhamnetin 3-O-glucoside | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Euonymus alatus |