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Literature summary for 2.4.1.91 extracted from

  • Hrazdina, G.
    Purification and properies of a UDPglucose: flavonoid 3-O-glucosyltransferase from Hippeastrum petals (1988), Biochim. Biophys. Acta, 955, 301-309.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
kaempferol
-
Hippeastrum sp.
0.07
-
quercetin about Hippeastrum sp.
0.07
-
cyanidin about Hippeastrum sp.
1
-
UDPglucose
-
Hippeastrum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
2 * 24500, SDS-PAGE Hippeastrum sp.
49000
-
gel filtration Hippeastrum sp.

Organism

Organism UniProt Comment Textmining
Hippeastrum sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hippeastrum sp.

Source Tissue

Source Tissue Comment Organism Textmining
petal
-
Hippeastrum sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Hippeastrum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + isosakuranetin at 11.2% the activity with kaempferol Hippeastrum sp. UDP + ?
-
?
UDP-glucose + pelargonidin at 4% the activity with kaempferol Hippeastrum sp. UDP + pelargonidin 3-O-glucoside
-
?
UDPglucose + apigenin at 4.2% the activity with kaempferol Hippeastrum sp. UDP + apigenin 3-O-glucoside
-
?
UDPglucose + cyanidin at 5% the activity with kaempferol Hippeastrum sp. UDP + cyanidin 3-O-glucoside
-
?
UDPglucose + dihydrokaempferol 9.6% of the activity with kaempferol Hippeastrum sp. UDP + dihydrokaempferol 3-O-glucoside
-
?
UDPglucose + dihydroquercetin
-
Hippeastrum sp. UDP + dihydroquercetin 3-O-glucoside
-
?
UDPglucose + kaempferol
-
Hippeastrum sp. UDP + kaempferol 3-O-glucoside
-
?
UDPglucose + malvidin at 4% the activity with kaempferol Hippeastrum sp. UDP + malvidin 3-O-glucoside
-
?
UDPglucose + naringenin at 3.2% the activity with kaempferol Hippeastrum sp. UDP + naringenin 3-O-glucoside
-
?
UDPglucose + quercetin 61.2% of the activity with kaempferol Hippeastrum sp. UDP + quercetin 3-O-glucoside
-
?

Subunits

Subunits Comment Organism
dimer 2 * 24500, SDS-PAGE Hippeastrum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Hippeastrum sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 60% of maximal activity, pH 9.0: about 70% of maximal activity Hippeastrum sp.