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Literature summary for 2.4.1.87 extracted from

  • Han, W.; Cai, L.; Wu, B.; Li, L.; Xiao, Z.; Cheng, J.; Wang, P.G.
    The wciN gene encodes an alpha-1,3-galactosyltransferase involved in the biosynthesis of the capsule repeating unit of Streptococcus pneumoniae serotype 6B (2012), Biochemistry, 51, 5804-5810.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
D102A inactive Streptococcus pneumoniae
D104A inactive Streptococcus pneumoniae
D116A the mutant remains active Streptococcus pneumoniae
D118A the mutant remains active Streptococcus pneumoniae
D28A the mutant remains active Streptococcus pneumoniae
D30A the mutant remains active Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38799
-
 x * 38799, calculated from amino acid sequence Streptococcus pneumoniae
39000
-
 x * 39000, SDS-PAGE Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
 serotype 6B
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity towards GlcNAcalpha-diphospho-O-(CH2)10CH3, UDP-D-glucose, Glcalpha-monophosphate, 4-nitrophenyl alpha-D-glucopyranoside, Glcalpha(1->2)Glc, Glcalpha-OMe, and Glcbeta-OMe Streptococcus pneumoniae ?
-
 ?
UDP-alpha-D-galactose + Glcalpha-diphospho-O(CH2)10CH3 wciN encodes an alpha-1,3-galactosyltransferase catalyzing the transfer of galactosyl from UDP-Gal onto the Glcalpha-diphosphate-lipid acceptor to form Galalpha(1->3)Glcalpha-diphosphate-lipid Streptococcus pneumoniae UDP + Galalpha(1->3)Glcalpha-diphospho-O(CH2)10CH3
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Streptococcus pneumoniae
? x * 38799, calculated from amino acid sequence Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
alpha-1,3-galactosyltransferase
-
 Streptococcus pneumoniae
WciN
-
 Streptococcus pneumoniae

General Information

General Information Comment Organism
physiological function the wciN gene encodes a galactosyltransferase involved in the repeating unit assembly of Pneumococcus type 6B capsular polysaccharide Streptococcus pneumoniae