Application | Comment | Organism |
---|---|---|
synthesis | attaching beta p-nitrophenyl to galactose converts the complex from a poor into a good substrate, the group mimics a monosaccharide like N-acetylglucosamine | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
5% PEG 6000, 0.1 M Tris-HCl, pH 8.0, 10 mM MnCl2, 10 mM UDP, in complex with substrate p-nitrophenyl-beta-galactoside, 25% glycerol as cryoprotectant | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | attaching beta p-nitrophenyl to galactose converts the complex from a poor into a good substrate, the group mimics a monosaccharide like N-acetylglucosamine | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
N-acetyllactosamine | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
1 | - |
p-nitrophenyl-beta-D-galactoside | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
107 | - |
p-nitrophenyl-alpha-D-galactoside | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
392 | - |
D-galactose | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + N-acetyllactosamine | Bos taurus | - |
UDP + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P14769 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + N-acetyllactosamine | - |
Bos taurus | UDP + ? | - |
? | |
UDP-galactose + D-galactose | - |
Bos taurus | UDP + alpha-D-galactosyl-1,3-D-galactoside | - |
? | |
UDP-galactose + N-acetyllactosamine | - |
Bos taurus | UDP + ? | - |
? | |
UDP-galactose + p-nitrophenyl-alpha-D-galactoside | - |
Bos taurus | UDP + ? | - |
? | |
UDP-galactose + p-nitrophenyl-beta-D-galactoside | - |
Bos taurus | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,3-galactosyltransferase | - |
Bos taurus |
alpha3GT | - |
Bos taurus |
UDP-galactose beta-galactosyl alpha-1,3-galactosyltransferase | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
p-nitrophenyl-alpha-D-galactoside | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
0.32 | - |
D-galactose | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
1.1 | - |
p-nitrophenyl-beta-D-galactoside | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
1.9 | - |
N-acetyllactosamine | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus |
General Information | Comment | Organism |
---|---|---|
metabolism | synthesis of biologically active oligosaccharides | Bos taurus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
p-nitrophenyl-alpha-D-galactoside | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
0.0008 | - |
D-galactose | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
1.1 | - |
p-nitrophenyl-beta-D-galactoside | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus | |
3.2 | - |
N-acetyllactosamine | 10 mM MnCl2, 0.3 mM UDP-galactose | Bos taurus |