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Literature summary for 2.4.1.87 extracted from

  • Jamaluddin, H.; Tumbale, P.; Ferns, T.A.; Thiyagarajan, N.; Brew, K.; Acharya, K.R.
    Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal) (2009), Biochem. Biophys. Res. Commun., 385, 601-604.
    View publication on PubMed

Application

Application Comment Organism
synthesis attaching beta p-nitrophenyl to galactose converts the complex from a poor into a good substrate, the group mimics a monosaccharide like N-acetylglucosamine Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
5% PEG 6000, 0.1 M Tris-HCl, pH 8.0, 10 mM MnCl2, 10 mM UDP, in complex with substrate p-nitrophenyl-beta-galactoside, 25% glycerol as cryoprotectant Bos taurus

Protein Variants

Protein Variants Comment Organism
additional information attaching beta p-nitrophenyl to galactose converts the complex from a poor into a good substrate, the group mimics a monosaccharide like N-acetylglucosamine Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
N-acetyllactosamine 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
1
-
p-nitrophenyl-beta-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
107
-
p-nitrophenyl-alpha-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
392
-
D-galactose 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2
-
Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactose + N-acetyllactosamine Bos taurus
-
UDP + ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus P14769
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactose + N-acetyllactosamine
-
Bos taurus UDP + ?
-
?
UDP-galactose + D-galactose
-
Bos taurus UDP + alpha-D-galactosyl-1,3-D-galactoside
-
?
UDP-galactose + N-acetyllactosamine
-
Bos taurus UDP + ?
-
?
UDP-galactose + p-nitrophenyl-alpha-D-galactoside
-
Bos taurus UDP + ?
-
?
UDP-galactose + p-nitrophenyl-beta-D-galactoside
-
Bos taurus UDP + ?
-
?

Synonyms

Synonyms Comment Organism
alpha-1,3-galactosyltransferase
-
Bos taurus
alpha3GT
-
Bos taurus
UDP-galactose beta-galactosyl alpha-1,3-galactosyltransferase
-
Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.059
-
p-nitrophenyl-alpha-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
0.32
-
D-galactose 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
1.1
-
p-nitrophenyl-beta-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
1.9
-
N-acetyllactosamine 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus

General Information

General Information Comment Organism
metabolism synthesis of biologically active oligosaccharides Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0006
-
p-nitrophenyl-alpha-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
0.0008
-
D-galactose 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
1.1
-
p-nitrophenyl-beta-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
3.2
-
N-acetyllactosamine 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus