Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and COS-7 cells as a His-tagged fusion protein | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
E317A | relative activity of mutant protein: 0.1% (compared to wild-type 100%) | Bos taurus |
E317C | relative activity of mutant protein: 0.8% (compared to wild-type 100%) | Bos taurus |
E317D | relative activity of mutant protein: 0.4% (compared to wild-type 100%) | Bos taurus |
E317H | relative activity of mutant protein: 0.1% (compared to wild-type 100%) | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
Gal-beta-1,4-GlcNAc-(CH2)8CO2CH3 | - |
Bos taurus | |
0.67 | - |
UDP-Gal | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P14769 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R | E317 is not the catalytic nucleophile proposed in the double-displacement mechanism. Either a different amino acid may act as the catalytic nucleophile or the reaction must proceed by a different mechanism | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activity: 100% wild-type, 0.1% mutant E317A, 0.4% mutant E317D, 0.8% mutant E317C, 0.1% mutant E317H | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-Gal + Gal-beta-1,4-GlcNAc-(CH2)8CO2CH3 | - |
Bos taurus | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,3galactosyltransferase | - |
Bos taurus |
alpha1,3GalT | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Bos taurus |