Application | Comment | Organism |
---|---|---|
biotechnology | integration of genes CYP79A1, CYP71E1, and UGT85B1 in Nicotiana tabacum chloroplast genome and functional expression, the enzymes convert endogenous tyrosine into dhurrin using electrons derived directly from the photosynthetic electron transport chain, without the need for the presence of an NADPH-dependent P450 oxidoreductase. The dhurrin produced in the engineered plants amounted to 0.1-0.2% of leaf dry weight compared to 6% in the origin Sorghum bicolor. Plant P450s involved in the synthesis of economically important compounds can be engineered into the thylakoid membrane of chloroplasts, and their full catalytic cycle can be driven directly by photosynthesis-derived electrons | Sorghum bicolor |
synthesis | integration of genes CYP79A1, CYP71E1, and UGT85B1 in Nicotiana tabacum chloroplast genome and functional expression, the enzymes convert endogenous tyrosine into dhurrin using electrons derived directly from the photosynthetic electron transport chain, without the need for the presence of an NADPH-dependent P450 oxidoreductase. The dhurrin produced in the engineered plants amounted to 0.1-0.2% of leaf dry weight compared to 6% in the origin Sorghum bicolor. Plant P450s involved in the synthesis of economically important compounds can be engineered into the thylakoid membrane of chloroplasts, and their full catalytic cycle can be driven directly by photosynthesis-derived electrons | Sorghum bicolor |
Cloned (Comment) | Organism |
---|---|
recombinant expression in Nicotiana tabacum chloroplasts, coexpression with CYP79A1 and CYP71E1 linked in a tricistronic operon construct with the essential regulatory elements | Sorghum bicolor |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering for introduction of the dhurrin biosynthesis pathway, starting with L-tyrosine and comprising the enzymes CYP79A1, CYP71E1, and UGT85B1 into Nicotiana tabacum. Integration of genes CYP79A1, CYP71E1, and UGT85B1 into a neutral site of the Nicotiana tabacum chloroplast genome and functional expression, overview. The enzymes convert endogenous tyrosine into dhurrin using electrons derived directly from the photosynthetic electron transport chain, without the need for the presence of an NADPH-dependent P450 oxidoreductase. The dhurrin produced in the engineered plants amounted to 0.1-0.2% of leaf dry weight compared to 6% in the origin Sorghum bicolor | Sorghum bicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast stroma | UGT85B1 is found mainly in the soluble stroma fraction | Sorghum bicolor | 9570 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose + (S)-4-hydroxymandelonitrile | Sorghum bicolor | - |
UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | i.e. dhurrin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | Q9SBL1 | gene UGT85B1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Sorghum bicolor | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UGT85B1 has a broad substrate specificity | Sorghum bicolor | ? | - |
? | |
UDP-D-glucose + (S)-4-hydroxymandelonitrile | - |
Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | i.e. dhurrin | ? |
General Information | Comment | Organism |
---|---|---|
metabolism | dhurrin is a cyanogenic glucoside and its synthesis from the amino acid tyrosine is catalysed by two membrane-bound cytochrome P450 enzymes (CYP79A1 and CYP71E1) and a soluble glucosyltransferase (UGT85B1), and is dependent on electron transfer from a P450 oxidoreductase | Sorghum bicolor |