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Literature summary for 2.4.1.85 extracted from
- The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. Isolation, cloning, heterologous expression, and substrate specificity (1999), J. Biol. Chem., 274, 35483-35491.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activates | Sorghum bicolor | |
| dithiothreitol | requirement, 5 mM | Sorghum bicolor |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of a full-length cDNA encoding enzyme, expression in Escherichia coli JM109, 492-amino acids translation product | Sorghum bicolor |
General Stability
| General Stability | Organism |
|---|---|
| activity is largely uneffected by both freezing at -80°C and addition of glycerol, DTT stabilizes | Sorghum bicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxybenzaldehyde | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
| 4-hydroxymandelonitrile | possibly an inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
| benzaldehyde | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
| Dhurrin | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
| mandelonitrile | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
| sambunigrin | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Sorghum bicolor | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52900 | - |
x * 52900, calculated from the amino acid sequence | Sorghum bicolor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + (S)-4-hydroxymandelonitrile | Sorghum bicolor | endogenous substrate is p-hydroxymandelonitrile, final step in the biosynthesis of dhurrin, transformation of the labile cyanohydrin into a stable storage form | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sorghum bicolor | Q9SBL1 | Pacific seeds, Australia, cultivar MR31 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 420fold, native and recombinant enzyme, expressed in Escherichia coli JM109 | Sorghum bicolor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | etiolated | Sorghum bicolor | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 2 days, lowering the concentration of DTT from 5 to 2 mM results in a 10fold decrease in activity | Sorghum bicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | exclusively specific for the presence of a benzyl group in the substrate, no substrate: acetone cyanohydrin, hydroquinone, p-hydroxybenzaldehyde, gentisic acid, caffeic acid, 2-hydroxy cinnamic acid, resveratrol, salicylic acid, p-hydroxymandelic acid, quercetin, caynidin, biochanin A, naringenin, apigenin, indole acetic acid, tomatidine | Sorghum bicolor | additional information | - |
? | |
| UDPalpha-D-glucose + benzoic acid | at 4% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + 1-O-benzoyl-beta-D-glucose | - |
? | |
| UDPglucose + (S)-4-hydroxymandelonitrile | C-terminal region encodes the UDPglucose binding domain | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | identical with dhurrin | ? | |
| UDPglucose + (S)-4-hydroxymandelonitrile | stereospecific for the S-enantiomer | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | identical with dhurrin | ? | |
| UDPglucose + (S)-4-hydroxymandelonitrile | endogenous substrate is p-hydroxymandelonitrile, final step in the biosynthesis of dhurrin, transformation of the labile cyanohydrin into a stable storage form | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | - |
? | |
| UDPglucose + (S)-mandelonitrile | at 78% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + (S)-mandelonitrile beta-D-glucoside | - |
? | |
| UDPglucose + benzyl alcohol | at 13% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + benzylglucoside | - |
? | |
| UDPglucose + geraniol | at 11% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + geraniol glucoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000-55000, SDS-PAGE | Sorghum bicolor |
| ? | x * 52900, calculated from the amino acid sequence | Sorghum bicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sbHMNGT | - |
Sorghum bicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sorghum bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
theoretical pI of 5.3 | Sorghum bicolor |
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