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Literature summary for 2.4.1.83 extracted from
- A thermostable dolichol phosphoryl mannose synthase responsible for glycoconjugate synthesis of the hyperthermophilic archaeon Pyrococcus horikoshii (2008), Extremophiles, 12, 665-676.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylcholine | relative activity: 122% | Pyrococcus horikoshii |  |
| phosphatidylethanolamine | relative activity: 125% | Pyrococcus horikoshii | |
| phosphatidylglycerol | relative activity: 356% | Pyrococcus horikoshii | |
| phosphatidylinositol | relative activity: 70% | Pyrococcus horikoshii | |
| phosphatidylserine | relative activity: 342% | Pyrococcus horikoshii | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length protein is expressed in Escherichia coli and the N-terminal domain is expressed in Saccharomyces cerevisiae | Pyrococcus horikoshii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D38A | 5.8% of the activity of wild-type | Pyrococcus horikoshii |
| D39A | 2% of the activity of wild-type | Pyrococcus horikoshii |
| D89A | 0.09% of the activity of wild-type | Pyrococcus horikoshii |
| D91A | 0.17% of the activity of wild-type | Pyrococcus horikoshii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylinositol | relative activity: 70% | Pyrococcus horikoshii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00059 | - |
C110-dolichyl phosphate | - |
Pyrococcus horikoshii | |
| 0.00117 | - |
C55-undecaprenyl phosphate | - |
Pyrococcus horikoshii | |
| 0.00159 | - |
C120-dolichyl phosphate | - |
Pyrococcus horikoshii | |
| 0.0023 | - |
C85-dolichyl phosphate | - |
Pyrococcus horikoshii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | N-terminal domain is membrane-bound | Pyrococcus horikoshii | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Pyrococcus horikoshii | |
| Mn2+ | required | Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41010 | - |
deduced from cDNA | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilized with Triton X-100 and purified to homogeneity | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + C110-dolichyl phosphate | - |
Pyrococcus horikoshii | GDP + C110-dolichyl D-mannosyl phosphate | - |
? | |
| GDP-mannose + C120-dolichyl phosphate | - |
Pyrococcus horikoshii | GDP + C120-dolichyl D-mannosyl phosphate | - |
? | |
| GDP-mannose + C55-undecaprenyl phosphate | - |
Pyrococcus horikoshii | GDP + C55-undecaprenyl D-mannosyl phosphate | - |
? | |
| GDP-mannose + C85-dolichyl phosphate | - |
Pyrococcus horikoshii | GDP + C85-dolichyl D-mannosyl phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dolichol phosphoryl mannose synthase | - |
Pyrococcus horikoshii |
| DPM synthase | - |
Pyrococcus horikoshii |
| PH0051 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
protein shows thermostability up to 75°C | Pyrococcus horikoshii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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