Cloned (Comment) | Organism |
---|---|
functionally active mutant enzyme S141A is expressed in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
S141A | functionally active mutant enzyme with more than 50% reduction in catalytic activity compared to wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the KM-values for dolichyl phosphate do not differ between the invitro phosphorylated and the control enzyme | Saccharomyces cerevisiae | |
0.0011 | - |
GDP-mannose | phosphorylated enzyme, 37°C, pH 7.0 | Saccharomyces cerevisiae | |
0.0012 | - |
GDP-mannose | non-phosphorylated enzyme, 37°C, pH 7.0 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P14020 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | in vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + dolichyl phosphate | - |
Saccharomyces cerevisiae | GDP + dolichyl D-mannosyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dol-P Man synthase | - |
Saccharomyces cerevisiae |
DPMS | - |
Saccharomyces cerevisiae |
mannosylphosphodolichol synthase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.1 | - |
GDP-mannose | non-phosphorylated enzyme, 37°C, pH 7.0 | Saccharomyces cerevisiae | |
70.9 | - |
GDP-mannose | phosphorylated enzyme, 37°C, pH 7.0 | Saccharomyces cerevisiae |