Literature summary for 2.4.1.83 extracted from

Banerjee, D.K.; Carrasquillo, E.A.; Hughey, P.; Schutzbach, J.S.; Martinez, J.A.; Baksi, K.
In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase (2005), J. Biol. Chem., 280, 4174-4181.

Search for more literature for 2.4.1.83

Cloned(Commentary)

Cloned (Comment)	Organism
functionally active mutant enzyme S141A is expressed in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
S141A	functionally active mutant enzyme with more than 50% reduction in catalytic activity compared to wild-type enzyme	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	the KM-values for dolichyl phosphate do not differ between the invitro phosphorylated and the control enzyme	Saccharomyces cerevisiae
0.0011	-	GDP-mannose	phosphorylated enzyme, 37°C, pH 7.0	Saccharomyces cerevisiae
0.0012	-	GDP-mannose	non-phosphorylated enzyme, 37°C, pH 7.0	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P14020	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	in vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-mannose + dolichyl phosphate	-	Saccharomyces cerevisiae	GDP + dolichyl D-mannosyl phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Dol-P Man synthase	-	Saccharomyces cerevisiae
DPMS	-	Saccharomyces cerevisiae
mannosylphosphodolichol synthase	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12.1	-	GDP-mannose	non-phosphorylated enzyme, 37°C, pH 7.0	Saccharomyces cerevisiae
70.9	-	GDP-mannose	phosphorylated enzyme, 37°C, pH 7.0	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)