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Literature summary for 2.4.1.83 extracted from

  • Villagomez-Castro, J.C.; Calvo-Mendez, C.; Vargas-Rodriguez, L.; Flores-Carreon, A.; Lopez-Romero, E.
    Entamoeba histolytica: solubilization and biochemical characterization of dolichol phosphate mannose synthase, an essential enzyme in glycoprotein biosynthesis (1998), Exp. Parasitol., 88, 111-120.
    View publication on PubMed
Search for more literature for 2.4.1.83

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Entamoeba histolytica
AMP
-
 Entamoeba histolytica
ATP
-
 Entamoeba histolytica
GDP strong Entamoeba histolytica
GMP
-
 Entamoeba histolytica
GTP strong Entamoeba histolytica
UDP
-
 Entamoeba histolytica
UMP
-
 Entamoeba histolytica
UTP
-
 Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 GDPmannose
-
 Entamoeba histolytica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required for dolichol phosphate mannose: 2.5-7.5 mM Mg2+ or 2-3 mM Mn2+. Mg2+ is a better activator than Mn2+ in the reverse reaction Entamoeba histolytica
Mn2+ divalent cation required for synthesis of dolichyl D-mannosyl phosphate: 2.5-7.5 mM Mg2+ or 2-3 mM Mn2+. Mg2+ is a better activator than Mn2+ in the reverse reaction Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDPmannose + dolichyl phosphate Entamoeba histolytica essential enzyme in glycoprotein biosynthesis GDP + dolichyl D-mannosyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDPmannose + dolichyl phosphate
-
 Entamoeba histolytica GDP + dolichyl D-mannosyl phosphate
-
 r
GDPmannose + dolichyl phosphate essential enzyme in glycoprotein biosynthesis Entamoeba histolytica GDP + dolichyl D-mannosyl phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 potassium phosphate or Tris-HCl buffer Entamoeba histolytica

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 80% of maximal activity, pH 8.5: about 54% of maximal activity Entamoeba histolytica