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Literature summary for 2.4.1.80 extracted from
- Drosophila glucosylceramide synthase: a negative regulator of cell death mediated by proapoptotic factors (2004), J. Biol. Chem., 279, 35995-36002.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GlcT-1, DNA sequence determination and analysis, functional expression in enzyme-deficient GM-95 cells, expression as V5/His-tagged protein in GM95 cells | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | loss of enzyme function by RNA interference leads to increased apoptotic cell death, conversely targeted expression of the enzyme can rescue cell death, overview | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | perinuclear and membranes | Drosophila melanogaster | 5783 | - |
| Golgi apparatus | membranes | Drosophila melanogaster | 5794 | - |
| membrane | enzyme contains a membrane-spanning region | Drosophila melanogaster | 16020 | - |
| additional information | N-terminal and TM regions of the enzyme are required for localization in the Golgi apparatus | Drosophila melanogaster | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Drosophila melanogaster | the enzyme has dual function as negative regulator of cell death mediated by proapoptotic factors and in catalyzing the formation of glucosylceramide, the core structure of major glycosphingolipids, ceramide generation might be a signal pathway | ? | - |
? |  |
| UDP-glucose + ceramide | Drosophila melanogaster | ceramide and its metabolites are important mediators of apoptosis and cell survival | UDP + glucosylceramide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
gene GlcT-1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | enzyme is expressed in all developmental stages | Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme has dual function as negative regulator of cell death mediated by proapoptotic factors and in catalyzing the formation of glucosylceramide, the core structure of major glycosphingolipids, ceramide generation might be a signal pathway | Drosophila melanogaster | ? | - |
? | |
| UDP-glucose + 6-([(N-7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproyl)sphingosine | - |
Drosophila melanogaster | UDP + N-(6-((4-nitrobenz-2-oxa-1,3-diazol-7-yl)amino)caproyl)-O1-D-glucosyl-sphingosine | - |
? | |
| UDP-glucose + ceramide | - |
Drosophila melanogaster | UDP + glucosylceramide | glucosylceramide is the core structure of major glycosphingolipids | ? | |
| UDP-glucose + ceramide | ceramide and its metabolites are important mediators of apoptosis and cell survival | Drosophila melanogaster | UDP + glucosylceramide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlcT-1 | - |
Drosophila melanogaster |
| glucosylceramide synthase | - |
Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 32 | assay at | Drosophila melanogaster |
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