Application | Comment | Organism |
---|---|---|
medicine | - |
Homo sapiens |
medicine | enzyme inhibition is a possible target for chemotherapeutic agents for a number of diseases, including cancer | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
cloning from rat brain genetic library and expression in Escherichia coli BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H169A | 26.4% activity compared to wild-type, reduced inhibition by diethyldicarbonate and increased protection by UDP-glucose | Rattus norvegicus |
H193A | 33.0% activity compared to wild-type, reduced inhibition by diethyldicarbonate and reduced protection by UDP-glucose | Rattus norvegicus |
H193D | no activity | Rattus norvegicus |
H193N | 23.0% activity compared to wild-type, reduced inhibition by diethyldicarbonate and reduced protection by UDP-glucose | Rattus norvegicus |
H193R | no activity | Rattus norvegicus |
H26A | 5.2% activity compared to wild-type | Rattus norvegicus |
H26D | 10.5% activity compared to wild-type | Rattus norvegicus |
H26N | 44.5% activity compared to wild-type | Rattus norvegicus |
H26R | 118.5% activity compared to wild-type, slightly enhanced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose | Rattus norvegicus |
H308A | 35.2% activity compared to wild-type, slightly reduced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose | Rattus norvegicus |
H308A/H309A | 10.8% activity compared to wild-type | Rattus norvegicus |
H309A | 69.1% activity compared to wild-type | Rattus norvegicus |
H322A | 3.8% activity compared to wild-type | Rattus norvegicus |
H322D | 2.6% activity compared to wild-type | Rattus norvegicus |
H322N | 49.8% activity compared to wild-type, slightly enhanced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose | Rattus norvegicus |
H322R | no activity | Rattus norvegicus |
H36A | 103.2% activity compared to wild-type, slightly enhanced inhibition by diethyldicarbonate and slightly reduced protection by UDP-glucose | Rattus norvegicus |
H90A | 119.3% activity compared to wild-type | Rattus norvegicus |
General Stability | Organism |
---|---|
glycerol stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | - |
Homo sapiens | |
D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | His193 mutants are not inhibited; i.e. PDMP | Rattus norvegicus | |
diethyl dicarbonate | i.e. DEPC; reversible by hydroxylamine, UDP-glucose protects, inhibitor acts on histidine residues, including His193, within or near UDP-glucose binding site | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
partial amino acid sequence alignment | Homo sapiens |
additional information | - |
partial amino acid sequence alignment | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16739 | - |
- |
Rattus norvegicus | Q9R0E0 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + an N-acylsphingosine = UDP + a beta-D-glucosyl-N-acylsphingosine | UDP-glucose and inhibitor D-PDMP binding region overlap and contain His193 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | i.e. ceramide | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | i.e. ceramide | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay | Rattus norvegicus | UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCS | - |
Homo sapiens |
GCS | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Homo sapiens |
7.4 | - |
assay at | Rattus norvegicus |