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Literature summary for 2.4.1.80 extracted from
- Glucosylceramide synthase activity in murine epidermis: quantitation, localization, regulation, and requirement for barrier homeostasis (1998), J. Lipid Res., 39, 277-285.
General Stability
| General Stability | Organism |
|---|---|
| no loss of activity during freezing and subsequent thawing | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | i.e. PDMP | Mus musculus |  |
| DL-threo-1-phenyl-2-hexadecanoyl-amino-3-pyrrolidino-1-propanol | i.e. most active PDMP-type congener, specific and reversible, in vivo and in vitro | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Mus musculus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + N-acylsphingosine | Mus musculus | enzyme is required for normal permeability barrier homeostasis | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | Mus musculus | initial key enzyme in glycosphingolipid biosynthesis | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
hairless mice strain Cr1:SKH1-hr | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | 80% of the activity in outer epidermis, 20% in lower epidermis | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000082 | - |
whole epidermis | Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 0.2 M MOPS, 0.25 M sucrose, pH 6.5, stable for more than 3 months | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + N-acylsphingosine | i.e. ceramide | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | enzyme is required for normal permeability barrier homeostasis | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
| UDP-glucose + N-acylsphingosine | initial key enzyme in glycosphingolipid biosynthesis | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlcCer synthase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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