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Literature summary for 2.4.1.80 extracted from

  • Coste, H.; Martel, M.B.; Azzar, G.; Got, R.
    UDPglucose-ceramide glucosyltransferase from porcine submaxillary glands is associated with the Golgi apparatus (1985), Biochim. Biophys. Acta, 814, 1-7.
    View publication on PubMed
Search for more literature for 2.4.1.80

Activating Compound

Activating Compound Comment Organism Structure
CHAPS i.e. 3-[(3-cholamidopropyl)dimethylamonio]-1propanesulfonate Sus scrofa
detergent CHAPS stimulates Sus scrofa
NADH omission in assay containing Golgi vesicles leads to loss of about 90% activity Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Sus scrofa
Triton X-100
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 UDP-glucose
-
 Sus scrofa
0.054
-
 ceramide
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Sus scrofa 5794
-
Golgi membrane
-
 Sus scrofa 139
-
membrane
-
 Sus scrofa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Sus scrofa
Mn2+ stimulates Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + N-acylsphingosine Sus scrofa catalyzes the first step during the sequential addition of carbohydrate moieties for ganglioside biosynthesis UDP + D-glucosyl-N-acylsphingosine
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
submaxillary ganglion
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000065
-
-
 Sus scrofa
0.0007
-
 Golgi fraction Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + N-acylsphingosine i.e. ceramide Sus scrofa UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine catalyzes the first step during the sequential addition of carbohydrate moieties for ganglioside biosynthesis Sus scrofa UDP + D-glucosyl-N-acylsphingosine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 sharp fall of activity below and above pH 6.5 Sus scrofa
6.5
-
-
 Sus scrofa