Cloned (Comment) | Organism |
---|---|
recombinnat expression in Escherichia coli | Bifidobacterium adolescentis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | formation of a glutaraldehyde cross-linked enzyme aggregate for high improvement of the enzyme's stability at 60°C, molecular imprinting of the cross-linked enzyme aggregate with a suitable substrate, i.e. glycerol, involving enzyme precipitation by tert-butyl alcohol can 2fold increase the transglucosylation activity, stability and specificity of the modified enzyme, method, overview. The modified enzyme is more useful as industrial biocatalyst than the native enzyme | Bifidobacterium adolescentis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Bifidobacterium adolescentis | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme partially from Escherichia coli via heat treatment removing the contaminating phosphatase activity | Bifidobacterium adolescentis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | - |
Bifidobacterium adolescentis | D-fructose + alpha-D-glucose 1-phosphate | - |
r |