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Literature summary for 2.4.1.56 extracted from

  • Jimenez, N.; Vilches, S.; Lacasta, A.; Regué, M.; Merino, S.; Tomás, J.
    A bifunctional enzyme in a single gene catalyzes the incorporation of GlcN into the Aeromonas core lipopolysaccharide (2009), J. Biol. Chem., 284, 32995-33005.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.56

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21 Aeromonas hydrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 lipopolysaccharide
-
 Aeromonas hydrophila
0.04
-
 UDP-N-acetyl-D-glucosamine
-
 Aeromonas hydrophila
0.052
-
 lipopolysaccharide mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila
0.119
-
 UDP-N-acetyl-D-glucosamine mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Tag affinity chromatography Aeromonas hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + lipopolysaccharide
-
 Aeromonas hydrophila ?
-
 ?

Synonyms

Synonyms Comment Organism
bifunctional glycosyltransferase
-
 Aeromonas hydrophila
WahA glycosyltransferase
-
 Aeromonas hydrophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 UDP-N-acetyl-D-glucosamine mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila
2.5
-
 lipopolysaccharide mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila
10
-
 UDP-N-acetyl-D-glucosamine
-
 Aeromonas hydrophila
25
-
 lipopolysaccharide
-
 Aeromonas hydrophila