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Literature summary for 2.4.1.46 extracted from

  • Miege, C.; Marechal, E.; Shimojima, M.; Awai, K.; Block, M.A.; Ohta, H.; Takamiya, K.I.; Douce, R.; Joyard, J.
    Biochemical and topological properties of type A MGDG synthase, a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG (1999), Eur. J. Biochem., 265, 990-1001.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression of isoenzyme MGD A in Escherichia coli Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide 0.15 mM, 65% inactivation after 10 min, recombinant isoenzyme MGD A, preincubation with diacylglycerol and/or phosphatidylglycerol protects Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast envelope inner membrane Spinacia oleracea 9941
-
chloroplast envelope monotopic membrane enzyme imbedded within 1 of the 2 leaflets Spinacia oleracea 9941
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, most likely a dimer, processed mature enzyme, immunoblot Spinacia oleracea
58000
-
 x * 58000, precursor, deduced from nucleotide sequence Spinacia oleracea
97000
-
 inactivation after gamma-ray irradiation Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea Q9SM44 isoenzyme MGD A
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + 1,2-diacylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, most likely a dimer, processed mature enzyme, immunoblot Spinacia oleracea
? x * 58000, precursor, deduced from nucleotide sequence Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 recombinant isoenzyme MGD A Spinacia oleracea