Application | Comment | Organism |
---|---|---|
synthesis | transglycosylation reactions with amylosucrase from Deinococcus geothermalis constitute an efficient and economical method to produce alpha-glucosyl flavonoids | Deinococcus geothermalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.31 | - |
6-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
3.9 | - |
6-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
4.67 | - |
4'-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
9.07 | - |
4'-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus geothermalis | Q1J0W0 | - |
- |
Deinococcus geothermalis DSM 11300 | Q1J0W0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + 4'-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and mono-hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis | D-fructose + ? | - |
? | |
sucrose + 4'-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and mono-hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis DSM 11300 | D-fructose + ? | - |
? | |
sucrose + 6-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and -hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis | D-fructose + ? | - |
? | |
sucrose + 6-hydroxyflavanone | 3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and -hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules | Deinococcus geothermalis DSM 11300 | D-fructose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASASE | - |
Deinococcus geothermalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Deinococcus geothermalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
4'-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
0.015 | - |
4'-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
0.71 | - |
6-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
0.84 | - |
6-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
4'-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
0.0032 | - |
4'-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis | |
0.1835 | - |
6-hydroxyflavanone | pH 5.0, 45°C | Deinococcus geothermalis | |
0.6943 | - |
6-hydroxyflavanone | pH 7.0, 45°C | Deinococcus geothermalis |