Cloned (Comment) | Organism |
---|---|
gene mfas, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Methylobacillus flagellatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74560 | - |
sequence calculation | Methylobacillus flagellatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylobacillus flagellatus | - |
gene mfas | - |
Methylobacillus flagellatus ATCC 51484 | - |
gene mfas | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Methylobacillus flagellatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the purified recombinant enzyme displays a typical amylosucrase activity by the demonstration of multiple activities of hydrolysis, isomerization, and polymerization. The enzyme also shows sucrose hydrolysis activity | Methylobacillus flagellatus | ? | - |
? | |
additional information | the purified recombinant enzyme displays a typical amylosucrase activity by the demonstration of multiple activities of hydrolysis, isomerization, and polymerization. The enzyme also shows sucrose hydrolysis activity | Methylobacillus flagellatus ATCC 51484 | ? | - |
? | |
sucrose + glycerol | glycerol is transglycosylated by the intermolecular transglycosylation activity of MFAS. The two major products are determined to be (2S)-1-O-alpha-D-glucosyl-glycerol or (2R)-1-O-alpha-D-glucosyl-glycerol, and 2-O-alpha-D-glucosyl-glycerol, in which a glucose molecule is linked to glycerol via an alpha-glycosidic linkage, NMR identification | Methylobacillus flagellatus | (2S)-1-O-alpha-D-glucosyl-glycerol + (2R)-1-O-alpha-D-glucosyl-glycerol + 2-O-alpha-D-glucosyl-glycerol | - |
? | |
sucrose + glycerol | glycerol is transglycosylated by the intermolecular transglycosylation activity of MFAS. The two major products are determined to be (2S)-1-O-alpha-D-glucosyl-glycerol or (2R)-1-O-alpha-D-glucosyl-glycerol, and 2-O-alpha-D-glucosyl-glycerol, in which a glucose molecule is linked to glycerol via an alpha-glycosidic linkage, NMR identification | Methylobacillus flagellatus ATCC 51484 | (2S)-1-O-alpha-D-glucosyl-glycerol + (2R)-1-O-alpha-D-glucosyl-glycerol + 2-O-alpha-D-glucosyl-glycerol | - |
? | |
sucrose + [(1->4)-alpha-D-glucosyl]n | the enzyme shows polymerization activity using sucrose as a sole substrate | Methylobacillus flagellatus | D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->4)-alpha-D-glucosyl]n | the enzyme shows polymerization activity using sucrose as a sole substrate | Methylobacillus flagellatus ATCC 51484 | D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASASE | - |
Methylobacillus flagellatus |
MFAS | - |
Methylobacillus flagellatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Methylobacillus flagellatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | activity range, profile overview | Methylobacillus flagellatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life of 19.3 min | Methylobacillus flagellatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Methylobacillus flagellatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | activity range, profile overview | Methylobacillus flagellatus |
General Information | Comment | Organism |
---|---|---|
evolution | the putative MFAS protein shares not only catalytic residues, but also five consensus regions (CR I-V), with other ASases, although the overall protein shows a low level of amino acid sequence homology when compared with known ASases (ACAS, AMAS, DGAS, DRAS, and NPAS), overview | Methylobacillus flagellatus |