Cloned (Comment) | Organism |
---|---|
- |
Neisseria polysaccharea |
Crystallization (Comment) | Organism |
---|---|
recombinant enzyme, equal amounts of 4 mg/ml enzyme in 150 mM NaCl, 50 mM Tris-HCl, pH 7.0, 1 mM EDTA and 1 mM dithiothreitol and reservoir solution consisting of 30% polyethylene glycol 6000 and 100 mM HEPES, pH 7.0, crystal structure at 1.4 A resolution | Neisseria polysaccharea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria polysaccharea | Q9ZEU2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Neisseria polysaccharea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | enzyme catalyzes both sucrose hydrolysis and oligosaccharide and polymer synthesis in the absence of an activator polymer | Neisseria polysaccharea | glucose + maltose + maltotriose + turanose + insoluble polymer | with 10 mM sucrose as the sole substrate, 30% glucose, 29% maltose, 18% maltotriose, 11% turanose and 12% insoluble polymer respectively | ? |