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Literature summary for 2.4.1.387 extracted from
- 6-alpha-Glucosyltransferase and 3-alpha-isomaltosyltransferase from Bacillus globisporus N75 (2003), J. Biosci. Bioeng., 95, 215-224 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sporosarcina globispora | Q84IQ3 | - |
- |
| Sporosarcina globispora N75 | Q84IQ3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sepabeads FP-DA13 column chromatography, and Sephacryl S-200 gel filtration, butyl-Toyopearl column chromatography, and SuperQ-Toyopearl 650C column chromatography | Sporosarcina globispora |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.32 | - |
culture supernatant, pH and temperature not specified in the publication | Sporosarcina globispora |
| 22.6 | - |
after 70.6fold purification, pH and temperature not specified in the publication | Sporosarcina globispora |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 112000, SDS-PAGE | Sporosarcina globispora |
| ? | x * 116392, calculated from amino acid sequence | Sporosarcina globispora |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-alpha-isomaltosyltransferase | - |
Sporosarcina globispora |
| IMT | - |
Sporosarcina globispora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Sporosarcina globispora |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
the thermal stability of the enzyme is 45°C at pH 6.0 for 60 min | Sporosarcina globispora |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
the enzyme is most active at pH 6.0-6.5 | Sporosarcina globispora |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | the enzyme is stable on the pH range of 4.5-10.0 in treatment at 4°C for 24h | Sporosarcina globispora |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sporosarcina globispora | isoelectric focusing | - |
4.2 |
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