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Literature summary for 2.4.1.387 extracted from

  • Kim, Y.; Kitaoka, M.; Hayashi, K.; Kim, C.; Cote, G.
    A synergistic reaction mechanism of a cycloalternan-forming enzyme and a D-glucosyltransferase for the production of cycloalternan in Bacillus sp. NRRL B-21195 (2003), Carbohydr. Res., 338, 2213-2220 .
    View publication on PubMed
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isomaltosyl maltooligosaccharide + an isomaltosyl residue Bacillus sp. NRRL B-21195
-
 alpha-isomaltosyl-(1->/3)-alpha-isomaltosyl-(1->/4)-maltooligosaccharide
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. NRRL B-21195
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, isomaltose-agarose column chromatography, DEAE Toyopearl 650S column chromatography, and MonoQ column chromatography Bacillus sp. NRRL B-21195

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.25
-
 purified enzyme, at pH 7.0 and 35°C Bacillus sp. NRRL B-21195

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 alpha-isomaltosyl-(1->4)-D-glucose
-
 Bacillus sp. NRRL B-21195 cycloalternan + 2 D-glucose
-
 ?
2 alpha-isomaltosyl-(1->4)-maltopentaose
-
 Bacillus sp. NRRL B-21195 cycloalternan + 2 maltopentaose
-
 ?
2 alpha-isomaltosyl-(1->4)-maltose
-
 Bacillus sp. NRRL B-21195 cycloalternan + 2 maltose
-
 ?
2 alpha-isomaltosyl-(1->4)-maltotetrase
-
 Bacillus sp. NRRL B-21195 cycloalternan + 2 maltotetraose
-
 ?
2 alpha-isomaltosyl-(1->4)-maltotriose
-
 Bacillus sp. NRRL B-21195 cycloalternan + 2 maltotriose
-
 ?
2 panose
-
 Bacillus sp. NRRL B-21195 2 D-glucose + ?
-
 ?
isomaltosyl maltooligosaccharide + an isomaltosyl residue
-
 Bacillus sp. NRRL B-21195 alpha-isomaltosyl-(1->/3)-alpha-isomaltosyl-(1->/4)-maltooligosaccharide
-
 ?

Subunits

Subunits Comment Organism
? x * 117000, SDS-PAGE Bacillus sp. NRRL B-21195

Synonyms

Synonyms Comment Organism
alternanase
-
 Bacillus sp. NRRL B-21195
CAFE
-
 Bacillus sp. NRRL B-21195
cycloalternan-forming enzyme
-
 Bacillus sp. NRRL B-21195