Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.387 extracted from

  • Nishimoto, T.; Aga, H.; Mukai, K.; Hashimoto, T.; Watanabe, H.; Kubota, M.; Fukuda, S.; Kurimoto, M.; Tsujisaka, Y.
    Purification and characterization of glucosyltransferase and glucanotransferase involved in the production of cyclic tetrasaccharide in Bacillus globisporus C11 (2002), Biosci. Biotechnol. Biochem., 66, 1806-1818 .
    View publication on PubMed
Search for more literature for 2.4.1.387

Metals/Ions

Metals/Ions Comment Organism Structure
additional information divalent cations have no effect on the stability or activity of the enzyme Sporosarcina globispora

Organism

Organism UniProt Comment Textmining
Sporosarcina globispora
-
-
-
Sporosarcina globispora C11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephabeads column chromatography, butyl-Toyopearl column chromatography, and Sephacryl gel filtration Sporosarcina globispora

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.167
-
 crude enzyme, at pH 6.0 and 35°C Sporosarcina globispora
22.9
-
 after 137fold purification, at pH 6.0 and 35°C Sporosarcina globispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-(3)3-isomaltosyl panose high activity Sporosarcina globispora ?
-
 ?
alpha-(3)3-isomaltosyl panose high activity Sporosarcina globispora C11 ?
-
 ?
alpha-(4)3-isomaltosyl panose low activity Sporosarcina globispora ?
-
 ?
alpha-(4)3-isomaltosyl panose low activity Sporosarcina globispora C11 ?
-
 ?
alpha-isomaltosyl beta-panoside low activity Sporosarcina globispora ?
-
 ?
alpha-isomaltosyl beta-panoside low activity Sporosarcina globispora C11 ?
-
 ?
alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-maltooligosaccharide
-
 Sporosarcina globispora cyclic tetrasaccharide + maltooligosaccharide
-
 ?
alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-maltooligosaccharide
-
 Sporosarcina globispora C11 cyclic tetrasaccharide + maltooligosaccharide
-
 ?
alpha-isomaltosyl-(1->4)-glucose high activity Sporosarcina globispora ?
-
 ?
alpha-isomaltosyl-(1->4)-maltose high activity Sporosarcina globispora ?
-
 ?
alpha-isomaltosyl-(1->4)-maltotetraose high activity Sporosarcina globispora ?
-
 ?
alpha-isomaltosyl-(1->4)-maltotriose high activity Sporosarcina globispora ?
-
 ?
alpha-isomaltosyl-maltooligosaccharide
-
 Sporosarcina globispora alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-maltooligosaccharide + ?
-
 ?
cyclo[->6]-alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->) very low activity Sporosarcina globispora ?
-
 ?
additional information no activity with methyl alpha-glucoside, methyl beta-glucoside, trehalose, kojibiose, nigerose, maltose, maltitol, maltotriose, maltotriitol, maltotetraose, maltopentaose, isopanose, isomaltose, neotrehalose, cellobiose, gentibiose, sucrose, erlose, alpha-maltosyl alpha-glucoside, alpha-isomaltosyl alpha-glucoside, alpha-isomaltotriosyl alpha-glucoside, alpha-isomaltosyl-(1->4)-sorbitol, alpha-isomaltosyl-(1->6)-glucose, alpha-cyclodextrin, beta-cyclodextrin, and gamma-cyclodextrin Sporosarcina globispora ?
-
-
additional information no activity with methyl alpha-glucoside, methyl beta-glucoside, trehalose, kojibiose, nigerose, maltose, maltitol, maltotriose, maltotriitol, maltotetraose, maltopentaose, isopanose, isomaltose, neotrehalose, cellobiose, gentibiose, sucrose, erlose, alpha-maltosyl alpha-glucoside, alpha-isomaltosyl alpha-glucoside, alpha-isomaltotriosyl alpha-glucoside, alpha-isomaltosyl-(1->4)-sorbitol, alpha-isomaltosyl-(1->6)-glucose, alpha-cyclodextrin, beta-cyclodextrin, and gamma-cyclodextrin Sporosarcina globispora C11 ?
-
-

Subunits

Subunits Comment Organism
? x * 102000, SDS-PAGE Sporosarcina globispora

Synonyms

Synonyms Comment Organism
CTS-producing enzyme
-
 Sporosarcina globispora
IMT
-
 Sporosarcina globispora
isomaltosyl transferase
-
 Sporosarcina globispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Sporosarcina globispora

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 the enzyme is stable on being heated to 40°C for 60 min Sporosarcina globispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Sporosarcina globispora

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9 the enzyme is stable from pH 4.5 to 9.0 for 60 min Sporosarcina globispora