Crystallization (Comment) | Organism |
---|---|
catalytic domain of R228K-Gal-T1 and mouse recombinant alpha-lactalbumin are cocrystallized in the presence of UDP-galactose and MnCl2. Crystals are grown at room temperature by the hanging drop method. Crystal structure of R228K-Gal-T1 complexed with alpha-lactalbumin, UDP-galactose, and Mn2+, determined at 1.9 Å resolution shows that the Asp318 side chain exhibits a minor alternate conformation, compared to that in the wild type | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
R228K | mutant enzyme shows 16% of the wild-type galactosyltransferase activity, mutation results in a 15fold higher glucosyltransferase activity, which is further enhanced by alpha-lactalbumin to nearly 25% of the galactosyltransferase activity of the wild type. The main effect of the mutation is on the kcat of glucosyltransferase, which increases 3-4fold, both in the absence and in the presence of alpha-lactalbumin simultaneously, the kcat for the galactosyltransferase reaction is reduced 30fold | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
N-acetylglucosamine | cosubstrate: UDP-galactose, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.0013 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.0025 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, in presence of alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.009 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.0105 | - |
UDP-galactose | cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.011 | - |
N-acetylglucosamine | cosubstrate: UDP-galactose, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.011 | - |
N-acetylglucosamine | cosubstrate: UDP-galactose, without alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.031 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.044 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.074 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, without alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.087 | - |
UDP-galactose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.091 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.093 | - |
UDP-galactose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.148 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, wild-type enzyme | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + N-acetylglucosamine | - |
Bos taurus | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
UDP-glucose + N-acetylglucosamine | reaction with 0.3% efficiency. R228K mutation results in a 15-fold higher glucosyltransferase activity, which is further enhanced by alpha-lactalbumin to nearly 25% of the galactosyltransferase activity of the wild type | Bos taurus | UDP + beta-D-glucosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(alpha4Gal-T1) | - |
Bos taurus |
alpha-1,4-galactosyltransferase I | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.06 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, without alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.17 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.17 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.25 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.25 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, in presence of alpha-lactalbumin, wild-type enzyme | Bos taurus | |
0.33 | - |
UDP-galactose | cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.33 | - |
N-acetylglucosamine | cosubstrate: UDP-galactose, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.52 | - |
UDP-galactose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.52 | - |
N-acetylglucosamine | cosubstrate: UDP-galactose, without alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.96 | - |
UDP-glucose | cosubstrate: N-acetylglucosamine, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
0.96 | - |
N-acetylglucosamine | cosubstrate: UDP-glucose, in presence of alpha-lactalbumin, mutant enzyme R228K | Bos taurus | |
14 | - |
UDP-galactose | cosubstrate: N-acetylglucosamine, without alpha-lactalbumin, wild-type enzyme | Bos taurus | |
14 | - |
N-acetylglucosamine | cosubstrate: UDP-galactose, without alpha-lactalbumin, wild-type enzyme | Bos taurus |