Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-lactalbumin | stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
- |
Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
Y289I | mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type | Bos taurus |
Y289L | mutation enhances GalNAc-transferase activity. Km for GlcNAc is incereased compared to the wild type | Bos taurus |
Y289N | mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-GalNAc + GlcNAc | transfer of GalNAc is only 1% of galactose transfer in wild type enzyme. Mutant enzyme Y289L exhibits nearly 100% of the galactose transferase activity | Bos taurus | ? | - |
? | |
UDPgalactose + glucose | in presence of alpha-lactalbumin | Bos taurus | lactose + UDP | - |
? | |
UDPgalactose + N-acetylglucosamine | - |
Bos taurus | UDP + N-acetyllactosamine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bos taurus |