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Literature summary for 2.4.1.38 extracted from
- Purification and characterization of recombinant human beta 1-4 galactosyltransferase expressed in Saccharomyces cerevisiae (1993), Eur. J. Biochem., 212, 113-120.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant enzyme is N-glycosylated | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
UDPgalactose | recombinant enzyme | Homo sapiens |  |
| 0.082 | - |
UDPgalactose | - |
Homo sapiens | |
| 2.8 | - |
N-acetylglucosamine | - |
Homo sapiens | |
| 3.6 | - |
N-acetylglucosamine | recombinant enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, deglycosylated enzyme form, SDS-PAGE | Homo sapiens |
| 48000 | - |
x * 48000, glycosylated enzyme form | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
expressed in Saccharomyces cerevisiae | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable fo at least 1 month | Homo sapiens |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, deglycosylated enzyme form, SDS-PAGE | Homo sapiens |
| ? | x * 48000, glycosylated enzyme form | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
inactivation at | Homo sapiens |
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Release 2023.1 (January 2023)
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