Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C80S/C196S | both the double and triple mutants show differing levels of disorder depending on their liganded state, with the level of disorder increasing when substrates are bound.The double mutant shows disorder over residues 177-180 in the unliganded and H-antigen bound forms, with disorder increasing to residues 176-185 for the UDP-, UDP + H-, and UDP-alpha-D-galactose + 3-deoxy-Gal inactive acceptor analog-bound structures. The double mutant has a reduction in Km for both donor and acceptor substrates. Mutation shows little effect over kcat | Homo sapiens |
C80S/C196S/C209S | both the double and triple mutants show differing levels of disorder depending on their liganded state, with the level of disorder increasing when substrates are bound.The unliganded triple mutant shows a nearly complete internal loop, with residues 176-181 disordered for the H-antigen-bound structure and 177-179 disordered for the UDP-bound structure. The UDP + H- and UDPGal + 3-deoxy-Gal inactive acceptor analog-bound structures of the triple mutant show an internal loop completely disordered over residues 176-184. The triple Cys-to-Ser mutant has an acceptor Km elevated approximately 5times over wild type, while the donor Km has doubled. Mutation shows little effect over kcat | Homo sapiens |
DELTA68-354 | construct in which the N-terminal transmembrane domain is deleted. Deletion results in a more crystallizable protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
UDP-GalNAc | mutant C80S/C196S, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.023 | - |
UDP-alpha-D-galactose | mutant C80S/C196S, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.032 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C80S/C196S, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose | Homo sapiens | |
0.045 | - |
UDP-GalNAc | deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.045 | - |
UDP-alpha-D-galactose | deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.055 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose | Homo sapiens | |
0.2 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc | Homo sapiens | |
0.3 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc | Homo sapiens | |
0.3 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C80S/C196S, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc | Homo sapiens | |
0.35 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose | Homo sapiens | |
0.78 | - |
UDP-GalNAc | mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.78 | - |
UDP-alpha-D-galactose | mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + UDP-GalNAc | - |
Homo sapiens | ? | - |
? | |
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
Homo sapiens | UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
blood group B glycosyltransferase GTB | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-GalNAc | Homo sapiens | |
0.41 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-GalNAc | Homo sapiens | |
0.7 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C80S/C196S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-GalNAc | Homo sapiens | |
5.6 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C80S/C196S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-alpha-D-galactose | Homo sapiens | |
7.3 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-alpha-D-galactose | Homo sapiens | |
8 | - |
beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-alpha-D-galactose | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |