Literature summary for 2.4.1.37 extracted from

Persson, M.; Letts, J.A.; Hosseini-Maaf, B.; Borisova, S.N.; Palcic, M.M.; Evans, S.V.; Olsson, M.L.
Structural effects of naturally occurring human blood group B galactosyltransferase mutations adjacent to the DXD motif (2007), J. Biol. Chem., 282, 9564-9570.

Search for more literature for 2.4.1.37

Protein Variants

Protein Variants	Comment	Organism
M214R	mutation is adjacent to the 211DVD213 motif. 1200fold decrease in kcat compared with wild type enzyme. The crystal structure of M214R shows that DVD motif coordination to Mn2+ is disrupted by Arg214 causing displacement of the metal by a water molecule. Individuals with the M214R mutation show the Bel variant expressing very low levels of B antigens	Homo sapiens
M214T	mutation is adjacent to the 211DVD213 motif. The crystal structure of the M214T mutant shows no change in DVD motif coordination to Mn2+. Instead a critical residue, Met266, which is responsible for determining donor specificity, has adopted alternate conformations. The conformation with the highest occupancy opens up the active site to accommodate the larger A-specific donor, UDP-GalNAc, accounting for the dual specificity. Individuals with M214T mutation give rise to AweakB phenotype	Homo sapiens
M214V	mutation is adjacent to the 211DVD213 motif. Individuals with M214T mutation give rise to AweakB phenotype	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	the enzyme has a characteristic 211DVD213 motif that coordinates to a Mn2+ ion shown to be critical in donor binding and catalysis	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P16442	recombinantly expressed in Escherichia coli	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-D-galactose + alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3	-	Homo sapiens	UDP + alpha-L-Fuc-(1,2)-[alpha-D-Galp-(1,3)-]-beta-D-Galp-O(CH2)7CH3	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00016	-	UDP-N-acetylgalactosamine	mutant enzyme M214R	Homo sapiens
0.0042	-	UDP-galactose	mutant enzyme M214R	Homo sapiens
0.37	-	UDP-N-acetylgalactosamine	mutant enzyme M214V	Homo sapiens
0.42	-	UDP-N-acetylgalactosamine	wild-type enzyme	Homo sapiens
1.3	-	UDP-galactose	mutant enzyme M214V	Homo sapiens
1.6	-	UDP-N-acetylgalactosamine	mutant enzyme M214T	Homo sapiens
5.1	-	UDP-galactose	wild-type enzyme	Homo sapiens
5.4	-	UDP-galactose	mutant enzyme M214T	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)