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Literature summary for 2.4.1.34 extracted from

  • Kamat, U.; Garg, R.; Sharma, C.B.
    Purification to homogeneity and characterization of a 1,3-beta-glucan (callose) synthase from germinating Arachis hypogaea cotyledons (1992), Arch. Biochem. Biophys., 298, 731-739.
    View publication on PubMed
Search for more literature for 2.4.1.34

Activating Compound

Activating Compound Comment Organism Structure
3-[(cholamidopropyl)dimethyl(ammonio)]propanesulfonate stimulate Arachis hypogaea
cellobiose no effect Arachis hypogaea
Digitonin stimulate Arachis hypogaea
GTP inhibits Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
1,2-Bis(O-aminophenoxy)ethane-N,N,N',N'-tetraacetate
-
 Arachis hypogaea
ATP
-
 Arachis hypogaea
CTP
-
 Arachis hypogaea
EDTA fully reversible by addition of Ca2+ Arachis hypogaea
EGTA fully reversible by addition of Ca2+ Arachis hypogaea
GTP
-
 Arachis hypogaea
Nonidet P-40
-
 Arachis hypogaea
octylglucoside
-
 Arachis hypogaea
Triton X-100
-
 Arachis hypogaea
UDP
-
 Arachis hypogaea
UMP
-
 Arachis hypogaea
UTP
-
 Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 UDPglucose
-
 Arachis hypogaea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Arachis hypogaea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Arachis hypogaea
Ca2+ optimum concentration 2-5 mM Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + (1,3-beta-D-glucosyl)n Arachis hypogaea synthesis of callose in higher plants, enzyme is latent in intact and undamaged cells, it is activated only under perturbed conditions, possible involvement in wound-healing process and in defense against pathogens, enzyme seems to play a vital role in a number of specialized developmental processes in plants, namely pollen maturation and sieve pore formation and gravitropism UDP + (1,3-beta-D-glucosyl)n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon germinating Arachis hypogaea
-

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 7.4, 1% digitonin, 0.01% 2-mercaptoethanol, 20% sucrose or glycerol, stable for at least 1 week, losing less than 10% of initial activity Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + (1,3-beta-D-glucosyl)n specific for UDPglucose as glucosyl donor Arachis hypogaea UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
UDPglucose + (1,3-beta-D-glucosyl)n synthesis of callose in higher plants, enzyme is latent in intact and undamaged cells, it is activated only under perturbed conditions, possible involvement in wound-healing process and in defense against pathogens, enzyme seems to play a vital role in a number of specialized developmental processes in plants, namely pollen maturation and sieve pore formation and gravitropism Arachis hypogaea UDP + (1,3-beta-D-glucosyl)n+1
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Arachis hypogaea

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 6.5 and 9: about 30% of activity maximum Arachis hypogaea

Cofactor

Cofactor Comment Organism Structure
ATP inhibits Arachis hypogaea