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Literature summary for 2.4.1.30 extracted from

  • Kitaoka, M.; Hayashi, K.
    Carbohydrate-processing phosphorolytic enzymes (2002), Trends Glycosci. Glycotechnol., 14, 35-50.
No PubMed abstract available
Search for more literature for 2.4.1.30

Activating Compound

Activating Compound Comment Organism Structure
additional information absolute requirement for sulfhydryl groups Euglena gracilis

Application

Application Comment Organism
synthesis synthesis of laminarioligosaccharides, degree of polymerization is dependent on the glucose/glucose 1-phosphate ratio, overview Euglena gracilis
synthesis synthesis of laminarioligosaccharides by combined action of laminaribiose phosphorylase EC 2.4.1.31 and beta-1,3-oligoglucan phosphorylase EC 2.4.1.30 Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate Euglena gracilis role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan (1,3-beta-D-glucosyl)n + phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-
Ochromonas malhamensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ochromonas malhamensis

Reaction

Reaction Comment Organism Reaction ID
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate inversion mechanism Euglena gracilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 Euglena gracilis (1,3-beta-D-glucosyl)n + phosphate
-
 r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 Ochromonas malhamensis (1,3-beta-D-glucosyl)n + phosphate
-
 r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan Euglena gracilis (1,3-beta-D-glucosyl)n + phosphate
-
 r
laminaribiose + phosphate
-
 Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
 r
laminaritriose + phosphate
-
 Euglena gracilis laminaribiose + alpha-D-glucose 1-phosphate
-
 r
paramylon + phosphate
-
 Ochromonas malhamensis ?
-
 r

Synonyms

Synonyms Comment Organism
laminaridextrin phosphorylase
-
 Euglena gracilis
laminaridextrin phosphorylase
-
 Ochromonas malhamensis
More EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificity, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase Euglena gracilis