Any feedback?
Literature summary for 2.4.1.279 extracted from
- Discovery of nigerose phosphorylase from Clostridium phytofermentans (2012), Appl. Microbiol. Biotechnol., 93, 1513-1522.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lachnoclostridium phytofermentans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose | - |
Lachnoclostridium phytofermentans |  |
| Co2+ | 1 mM, 80% inhibition | Lachnoclostridium phytofermentans | |
| Cu2+ | 1 mM, 98.7% inhibition | Lachnoclostridium phytofermentans | |
| Fe2+ | 1 mM, 68% inhibition | Lachnoclostridium phytofermentans | |
| additional information | no inhibition by EDTA | Lachnoclostridium phytofermentans | |
| Ni2+ | 1 mM, 95.4% inhibition | Lachnoclostridium phytofermentans | |
| Zn2+ | 1 mM, 85% inhibition | Lachnoclostridium phytofermentans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
phosphate | pH 7.0, 30°C, phosphorolysis reaction, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans | |
| 1.7 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolyis reaction | Lachnoclostridium phytofermentans | |
| 3.3 | - |
D-glucose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 79 | - |
1,5-anhydro-D-glucitol | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 86 | - |
D-xylose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 104 | - |
D-galactose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Lachnoclostridium phytofermentans | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no divalent metal ions required | Lachnoclostridium phytofermentans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87170 | - |
calculated from amino acid sequence | Lachnoclostridium phytofermentans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans | Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans ATCC 700394 | Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lachnoclostridium phytofermentans | A9KT32 | - |
- |
| Lachnoclostridium phytofermentans ATCC 700394 | A9KT32 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate = D-glucose + beta-D-glucose 1-phosphate | sequential bi-bi mechanism | Lachnoclostridium phytofermentans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
pH 7.0, 30°C, phosphorolysis reaction, substrate 2-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans |
| 0.17 | - |
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + methyl alpha-D-glucoside | Lachnoclostridium phytofermentans |
| 4.6 | - |
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-galactose | Lachnoclostridium phytofermentans |
| 8.1 | - |
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol | Lachnoclostridium phytofermentans |
| 9.3 | - |
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-xylose | Lachnoclostridium phytofermentans |
| 32 | - |
pH 7.0, 30°C, phosphorolysis reaction, substrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans |
| 54 | - |
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-glucose | Lachnoclostridium phytofermentans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose | Lachnoclostridium phytofermentans | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| 2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose | Lachnoclostridium phytofermentans ATCC 700394 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. | Lachnoclostridium phytofermentans | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | phosphorolysis reaction with nigerose | Lachnoclostridium phytofermentans | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. | Lachnoclostridium phytofermentans ATCC 700394 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | phosphorolysis reaction with nigerose | Lachnoclostridium phytofermentans ATCC 700394 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol | reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate | - |
? | |
| beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol | reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans ATCC 700394 | 3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate | - |
? | |
| beta-D-glucose 1-phosphate + D-galactose | reverse phosphorolysis reaction 8.5% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose, no significant preference for specific anomer of the acceptor | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate | - |
? | |
| beta-D-glucose 1-phosphate + D-glucose | reverse phosphorolysis reaction, no significant preference for specific anomer of the acceptor | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
| beta-D-glucose 1-phosphate + D-xylose | reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-xylopyranose + 2-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate | products are formed in the ratio 63:37% | ? | |
| beta-D-glucose 1-phosphate + methyl alpha-D-glucoside | reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans | methyl-3-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + methyl-2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate | products are formed in the ratio 71:29% | ? | |
| additional information | in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine | Lachnoclostridium phytofermentans | ? | - |
? | |
| additional information | in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine | Lachnoclostridium phytofermentans ATCC 700394 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cphy1874 | - |
Lachnoclostridium phytofermentans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Lachnoclostridium phytofermentans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67 | - |
phosphate | pH 7.0, 30°C, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose | Lachnoclostridium phytofermentans | |
| 69 | - |
D-galactose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 110 | - |
D-glucose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 118 | - |
D-xylose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 120 | - |
1,5-anhydro-D-glucitol | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Lachnoclostridium phytofermentans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Lachnoclostridium phytofermentans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.4 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C | Lachnoclostridium phytofermentans | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
D-galactose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 1.4 | - |
D-xylose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 1.5 | - |
1,5-anhydro-D-glucitol | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 33 | - |
D-glucose | pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
