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Literature summary for 2.4.1.264 extracted from

  • Vojnov, A.A.; Bassi, D.E.; Daniels, M.J.; Dankert, M.A.
    Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris (2002), Carbohydr. Res., 337, 315-326.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol structural analysis of the trisaccharide accumulating in mutatants with an insertion in the gumL gene Xanthomonas campestris UDP + GlcA-beta-(1-2)-D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
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assay at Xanthomonas campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
assay at Xanthomonas campestris

General Information

General Information Comment Organism
malfunction a transposon responsible for the mutation is located within gumK gene. The Tn5 insertion mutant with a reduced slimy phenotype fails to produce the pentasaccharide repeating-unit of xanthan. Only three sugars are transferred to the prenyl phosphate intermediate. The lipid-associated saccharide is the trisaccharide reducing end of the pentasaccharide from the wild-type strain. This trisaccharide is built up from UDP-Glc and GDP-Man, and a glucose residue is at the reducing end, linked to an allylic prenol through a diphosphate bridge. the trisaccharide-PP-polyprenol is the precursor of the polymer. This new polymer, a polytrisaccharide, is detected also in vivo. A recombinant plasmid with the whole gum cluster restored the wild type phenotype Xanthomonas campestris