Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion crystallisation experiments are performed | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
hOGT (26-end) | mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-[hydroxy(phosphonomethyl)phosphoryl]uridine | non-hydrolysable alpha,beta-methylene bisphosphonate analogue with the diphosphate oxygen replaced by a methylene group | Homo sapiens | |
alloxan | low micromolar inhibitor | Homo sapiens | |
additional information | testing UDP-GlcNAc/UDP analogues and evaluate their inhibitory properties and structural binding modes in vitro. These analogues are not active on living cells, they inhibit the enzyme in the micromolar range and together with the structural data provide useful templates for further optimisation | Homo sapiens | |
UDP | product inhibition | Homo sapiens | |
UDP-1-deoxy-1-methylene-N-acetyl-alpha-D-glucosamine | weak hOGT inhibitor | Homo sapiens | |
UDP-1-deoxy-1-thio-N-acetyl-alpha-D-glucosamine | a sub-millimolar inhibitor of hOGT and substrate binding probe | Homo sapiens | |
UDP-S-GlcNAc | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic experiments using recombinant hOGT/XcOGT | Homo sapiens | |
0.001 | - |
UDP-GlcNAc | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme transfers GlcNAc onto substrate proteins using UDP-GlcNAc as the sugar donor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme transfers GlcNAc onto substrate proteins using UDP-GlcNAc as the sugar donor | Homo sapiens | ? | - |
? | |
additional information | UDP-1-deoxy-1-thio-N-acetyl-alpha-D-glucosamine is no substrate | Homo sapiens | ? | - |
? | |
YSDSPSTST + UDP-GlcNAc | - |
Homo sapiens | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hOGT | - |
Homo sapiens |
O-GlcNAc transferase | - |
Homo sapiens |
OGT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
hOGT assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0018 | - |
- |
Homo sapiens | UDP | |
0.009 | - |
- |
Homo sapiens | 5'-O-[hydroxy(phosphonomethyl)phosphoryl]uridine | |
0.018 | - |
- |
Homo sapiens | alloxan | |
0.041 | - |
- |
Homo sapiens | UDP-1-deoxy-1-methylene-N-acetyl-alpha-D-glucosamine | |
0.093 | - |
- |
Homo sapiens | UDP-1-deoxy-1-thio-N-acetyl-alpha-D-glucosamine |