Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | nearly all of the peptides became better acceptors as the concentration of UDP-GlcNAc increased with the exception of the YSPTSPSYSPTSPS | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
using the insect cell baculovirus system to overexpress p110 subunit | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic studies suggest that OGT functions through a random bi-bi kinetic mechanism and that the protein has three distinct binding constants for the UDPGlcNAc sugar donor | Rattus norvegicus | |
0.006 | - |
UDP-GlcNAc | calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer | Rattus norvegicus | |
0.006 | - |
UDP-GlcNAc | D6 monomer | Rattus norvegicus | |
0.035 | - |
UDP-GlcNAc | calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer | Rattus norvegicus | |
0.06 | - |
UDP-GlcNAc | D6 monomer | Rattus norvegicus | |
0.107 | - |
PGGSTPVSSANMM | His-p110 trimer | Rattus norvegicus | |
0.215 | - |
PGGSTPVSSANMM | D6 monomer | Rattus norvegicus | |
0.217 | - |
UDP-GlcNAc | calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
nucleus | - |
Rattus norvegicus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | p110 subunit of the enzyme forms both homo- and heterotrimers that appear to have different binding affinities for UDP-GlcNAc | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity chromatography, purified p110 subunit | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of OGT on acceptor substrates is dependent on UDP-GlcNAc concentrations. The observation that OGT has three separate binding constants for UDP-GlcNAc suggested that UDP-GlcNAc concentration could directly affect OGT activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | p110 subunit of the enzyme forms both homo- and heterotrimers that appear to have different binding affinities for UDP-GlcNAc | Rattus norvegicus | ? | - |
? | |
UDP-GlcNAc + AIPVSREEK | - |
Rattus norvegicus | UDP + AIPV-(GlcNAc)SREEK | - |
? | |
UDP-GlcNAc + ITISETPSSTTTTQITK | - |
Rattus norvegicus | UDP + ITI-(GlcNAc)SETPSSTTTTQITK | - |
? | |
UDP-GlcNAc + KKFELLPTPPLSPSRR | - |
Rattus norvegicus | UDP + KKFELLP-(GlcNAc)TPPLSPSRR | - |
? | |
UDP-GlcNAc + PGGSTPVSSANMM | PGGSTPVSSANMM is the best acceptor | Rattus norvegicus | UDP + PGGSTPV-(GlcNAc)SSANMM | - |
? | |
UDP-GlcNAc + YSDSPSTST | - |
Rattus norvegicus | UDP + ? | - |
? | |
UDP-GlcNAc + YSPTSPSYSPTSPS | YSPTSPSYSPTSPS is a poor substrate | Rattus norvegicus | UDP + Y-(GlcNAc)SPT-(GlcNAc)SPSYSPT-(GlcNAc)SPS | - |
? | |
UDP-GlcNAc + [YSPTSPSYSPTSPS]5 | - |
Rattus norvegicus | UDP + [Y-(GlcNAc)SP-(GlcNAc)TSPSYSP-(GlcNAc)TSPS]5 | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | kinetic analyses of the full-length trimer and the truncated monomer forms of OGT | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAc transferase | - |
Rattus norvegicus |
OGT | - |
Rattus norvegicus |