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Literature summary for 2.4.1.255 extracted from

  • Kreppel, L.K.; Hart, G.W.
    Regulation of a cytosolic and nuclear O-GlcNAc transferase. Role of the tetratricopeptide repeats (1999), J. Biol. Chem., 274, 32015-32022.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information nearly all of the peptides became better acceptors as the concentration of UDP-GlcNAc increased with the exception of the YSPTSPSYSPTSPS Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
using the insect cell baculovirus system to overexpress p110 subunit Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic studies suggest that OGT functions through a random bi-bi kinetic mechanism and that the protein has three distinct binding constants for the UDPGlcNAc sugar donor Rattus norvegicus
0.006
-
UDP-GlcNAc calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer Rattus norvegicus
0.006
-
UDP-GlcNAc D6 monomer Rattus norvegicus
0.035
-
UDP-GlcNAc calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer Rattus norvegicus
0.06
-
UDP-GlcNAc D6 monomer Rattus norvegicus
0.107
-
PGGSTPVSSANMM His-p110 trimer Rattus norvegicus
0.215
-
PGGSTPVSSANMM D6 monomer Rattus norvegicus
0.217
-
UDP-GlcNAc calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-
nucleus
-
Rattus norvegicus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus p110 subunit of the enzyme forms both homo- and heterotrimers that appear to have different binding affinities for UDP-GlcNAc ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography, purified p110 subunit Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of OGT on acceptor substrates is dependent on UDP-GlcNAc concentrations. The observation that OGT has three separate binding constants for UDP-GlcNAc suggested that UDP-GlcNAc concentration could directly affect OGT activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information p110 subunit of the enzyme forms both homo- and heterotrimers that appear to have different binding affinities for UDP-GlcNAc Rattus norvegicus ?
-
?
UDP-GlcNAc + AIPVSREEK
-
Rattus norvegicus UDP + AIPV-(GlcNAc)SREEK
-
?
UDP-GlcNAc + ITISETPSSTTTTQITK
-
Rattus norvegicus UDP + ITI-(GlcNAc)SETPSSTTTTQITK
-
?
UDP-GlcNAc + KKFELLPTPPLSPSRR
-
Rattus norvegicus UDP + KKFELLP-(GlcNAc)TPPLSPSRR
-
?
UDP-GlcNAc + PGGSTPVSSANMM PGGSTPVSSANMM is the best acceptor Rattus norvegicus UDP + PGGSTPV-(GlcNAc)SSANMM
-
?
UDP-GlcNAc + YSDSPSTST
-
Rattus norvegicus UDP + ?
-
?
UDP-GlcNAc + YSPTSPSYSPTSPS YSPTSPSYSPTSPS is a poor substrate Rattus norvegicus UDP + Y-(GlcNAc)SPT-(GlcNAc)SPSYSPT-(GlcNAc)SPS
-
?
UDP-GlcNAc + [YSPTSPSYSPTSPS]5
-
Rattus norvegicus UDP + [Y-(GlcNAc)SP-(GlcNAc)TSPSYSP-(GlcNAc)TSPS]5
-
?

Subunits

Subunits Comment Organism
trimer kinetic analyses of the full-length trimer and the truncated monomer forms of OGT Rattus norvegicus

Synonyms

Synonyms Comment Organism
O-GlcNAc transferase
-
Rattus norvegicus
OGT
-
Rattus norvegicus