Cloned (Comment) | Organism |
---|---|
genes agtA and agtB, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression in Aspergillus niger strain MGG029, subcloning in Escherichia coli | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an agtA knockout of Aspergillus niger shows an increased susceptibility towards the cell wall-disrupting compound, phenotypic characterization of CFW hypersensitive DELTAagtA strain and AgtA/AgtB overexpression strains, overview | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | associated | Aspergillus niger | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
strains CBS 513.88, NRRL3122, and N402, isozymes AgtA and AgtB encoded by genes agtA and agtB | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycolipoprotein | the enzyme is cell wall-associated via glycosylphosphatidylinositol anchoring | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | both isozymes AgtA and AgtB show transglycosylation activity on donor substrates with alpha-(1,4)-glycosidic bonds and at least five anhydroglucose units forming alpha-(1,4)-glycosidic bonds. Their reaction products reach a degree of polymerization of at least 30. Maltose and larger maltooligosaccharides are the most efficient acceptor substrates, although AgtA also uses small nigerooligosaccharides containing alpha-(1,3)-glycosidic bonds as acceptor substrate, reaction products, overview. The enzyme also shows hydrolyzing activity with potato starch | Aspergillus niger | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AgtA | - |
Aspergillus niger |
AgtB | - |
Aspergillus niger |
More | the enzyme belongs to the alpha-amylase family | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 8 | - |
Aspergillus niger |