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Literature summary for 2.4.1.243 extracted from
- Molecular and functional characterization of novel fructosyltransferases and invertases from Agave tequilana (2012), PLoS ONE, 7, e35878.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes Atq6G-FFT-1 and -2, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X-33 | Agave tequilana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69700 | - |
x * 70700, about, Atq6G-FFT-1, sequence calculation, x * 69700, about, Atq6G-FFT-2, sequence calculation | Agave tequilana |
| 70700 | - |
x * 70700, about, Atq6G-FFT-1, sequence calculation, x * 69700, about, Atq6G-FFT-2, sequence calculation | Agave tequilana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agave tequilana | - |
genes Atq6G-FFT-1 and -2 | - |
| Agave tequilana azul | - |
genes Atq6G-FFT-1 and -2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | Atq6G-FFT-1 and Atq6G-FFT-2 contains each seven glycosylation sites | Agave tequilana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | age-related expression of Atq6G-FFT-1 in 5 and 7 year old plants, overview | Agave tequilana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose | - |
Agave tequilana | ? | - |
? |  |
| 1-kestose | - |
Agave tequilana azul | ? | - |
? | |
| 1-kestose + 3 sucrose | - |
Agave tequilana | 3 neokestose + H2O | plus a 4c tetrasaccharide moiety | ? | |
| 1-kestose + 3 sucrose | - |
Agave tequilana azul | 3 neokestose + H2O | plus a 4c tetrasaccharide moiety | ? | |
| additional information | the Atq6GFFT-1 consensus sequence is DPCG, Atq6GFFT-1 and Atq6G-1FFT-2 utilize primarily 1-kestose as a substrate, a Asn/Trp couple is involved in substrate recognition | Agave tequilana | ? | - |
? | |
| additional information | the Atq6GFFT-1 consensus sequence is DPCG, Atq6GFFT-1 and Atq6G-1FFT-2 utilize primarily 1-kestose as a substrate, a Asn/Trp couple is involved in substrate recognition | Agave tequilana azul | ? | - |
? | |
| sucrose | - |
Agave tequilana | neokestose | - |
? | |
| sucrose | - |
Agave tequilana azul | neokestose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70700, about, Atq6G-FFT-1, sequence calculation, x * 69700, about, Atq6G-FFT-2, sequence calculation | Agave tequilana |
| More | Atq6G-FFT-1 and Atq6G-FFT-2 sequence comparisons | Agave tequilana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6G-FFT | - |
Agave tequilana |
| Atq6G-FFT-1 | - |
Agave tequilana |
| Atq6G-FFT-2 | - |
Agave tequilana |
| fructan: fructan 6G-fructosyltransferase | - |
Agave tequilana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Agave tequilana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Agave tequilana | Atq6G-FFT-2, sequence calculation | - |
5.11 |
| Agave tequilana | Atq6G-FFT-1, sequence calculation | - |
5.57 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is required for fructan biosynthesis | Agave tequilana |
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Release 2023.1 (January 2023)
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