Cloned (Comment) | Organism |
---|---|
overexpression of the His-tagged GBSSI catalytic domain, residues Met83-Pro609, in Escherichia coli strain BL21 | Oryza sativa |
Crystallization (Comment) | Organism |
---|---|
recombinant His-tagged GBSSI catalytic domain, residues Met83-Pro609, free or in complex with ADP-glucose, sitting drop vapour diffusion method, mixing of 0.0003 ml of 6 mg/ml protein in 20 mM Tris, pH 8.0, and 0.2 M NaCl, with 0.0003 m of reservoir solution, containing 1.5 M lithium sulfate and 0.1 M HEPES-sodium, pH 7.5, and equilibration against 0.05 ml of reservoir solution, 20°C, 4 weeks, X-ray diffraction structure determination and analysis at 2.7-3.0 A resolution | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, recombinant His-tagged catalytic domain comprising residues Met83-Pro609, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | B7EHV0 | var. japonica | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged GBSSI catalytic domain, residues Met83-Pro609, from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Oryza sativa |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, recombinant His-tagged catalytic domain comprising residues Met83-Pro609, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
GBSSI | - |
Oryza sativa |
granule bound starch synthase I | - |
Oryza sativa |
Waxy protein | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
additional information | three-dimensional structures of free and ADP-glucose bound catalytic domain, overview | Oryza sativa |
physiological function | the waxy protein GBSSI, encoded by the wx locus, is an enzyme essential for amylose synthesis | Oryza sativa |