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Literature summary for 2.4.1.242 extracted from

  • Momma, M.; Fujimoto, Z.
    Interdomain disulfide bridge in the rice granule bound starch synthase I catalytic domain as elucidated by X-ray structure analysis (2012), Biosci. Biotechnol. Biochem., 76, 1591-1595.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the His-tagged GBSSI catalytic domain, residues Met83-Pro609, in Escherichia coli strain BL21 Oryza sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant His-tagged GBSSI catalytic domain, residues Met83-Pro609, free or in complex with ADP-glucose, sitting drop vapour diffusion method, mixing of 0.0003 ml of 6 mg/ml protein in 20 mM Tris, pH 8.0, and 0.2 M NaCl, with 0.0003 m of reservoir solution, containing 1.5 M lithium sulfate and 0.1 M HEPES-sodium, pH 7.5, and equilibration against 0.05 ml of reservoir solution, 20°C, 4 weeks, X-ray diffraction structure determination and analysis at 2.7-3.0 A resolution Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, recombinant His-tagged catalytic domain comprising residues Met83-Pro609, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa B7EHV0 var. japonica
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged GBSSI catalytic domain, residues Met83-Pro609, from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Oryza sativa

Subunits

Subunits Comment Organism
? x * 59000, recombinant His-tagged catalytic domain comprising residues Met83-Pro609, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
GBSSI
-
Oryza sativa
granule bound starch synthase I
-
Oryza sativa
Waxy protein
-
Oryza sativa

General Information

General Information Comment Organism
additional information three-dimensional structures of free and ADP-glucose bound catalytic domain, overview Oryza sativa
physiological function the waxy protein GBSSI, encoded by the wx locus, is an enzyme essential for amylose synthesis Oryza sativa