Cloned (Comment) | Organism |
---|---|
och1+ gene, sequencing | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption mutant of och1+: viable, slow-growing phenotype, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase | Schizosaccharomyces pombe |
W266stop | inactive mutant strain 7X4 with a nonsense mutation resulting in a truncated Och1p, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Schizosaccharomyces pombe | SpOch1p is a key enzyme in outer chain elongation of N-linked oligosaccharides on glycoproteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | Q9UTR6 | strains JY741 and L972 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GDP-mannose + Man8GlcNAc = GDP + Man9GlcNAc | requires Mn2+, in Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides, Man8GlcNAc and Man9GlcNAc are equally good substrates | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + pyridylaminated Man5GlcNAc2 | incorporates alpha-1,6-linked mannose, poor substrate | Schizosaccharomyces pombe | GDP + pyridylaminated Man6GlcNAc2 | - |
? | |
GDP-mannose + pyridylaminated Man6GlcNAc2 | incorporates alpha-1,6-linked mannose | Schizosaccharomyces pombe | GDP + pyridylaminated Man7GlcNAc2 | - |
? | |
GDP-mannose + pyridylaminated Man7GlcNAc2 | incorporates alpha-1,6-linked mannose | Schizosaccharomyces pombe | GDP + pyridylaminated Man8GlcNAc2 | - |
? | |
GDP-mannose + pyridylaminated Man8GlcNAc2 | incorporates alpha-1,6-linked mannose | Schizosaccharomyces pombe | GDP + pyridylaminated Man9GlcNAc2 | - |
? | |
GDP-mannose + pyridylaminated Man9GlcNAc2 | incorporates alpha-1,6-linked mannose | Schizosaccharomyces pombe | GDP + pyridylaminated Man10GlcNAc2 | - |
? | |
GDP-mannose + pyridylaminated oligosaccharide acceptor | incorporates alpha-1,6-linked mannose, substrate specificity | Schizosaccharomyces pombe | GDP + pyridylaminated alpha-mannosyl-1,6-oligosaccharide | - |
? | |
additional information | SpOch1p has a wider substrate specificity than ScOch1p from Saccharomyces cerevisiae | Schizosaccharomyces pombe | ? | - |
? | |
additional information | SpOch1p is a key enzyme in outer chain elongation of N-linked oligosaccharides on glycoproteins | Schizosaccharomyces pombe | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,6-mannosyltransferase | - |
Schizosaccharomyces pombe |
GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase | - |
Schizosaccharomyces pombe |
glycolipid 6-alpha-mannosyltransferase | - |
Schizosaccharomyces pombe |
glycoprotein alpha1-6-mannosyltransferase | - |
Schizosaccharomyces pombe |
mannosyltransferase, guanosine diphosphomannose-glycoprotein alpha1-6- | - |
Schizosaccharomyces pombe |
och1+ | - |
Schizosaccharomyces pombe |
SpOch1p | och1+ gene product, Schizosaccharomyces pombe | Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Schizosaccharomyces pombe |