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Literature summary for 2.4.1.224 extracted from

  • Kitagawa, H.; Egusa, N.; Tamura, J.I.; Kusche-Gullberg, M.; Lindahl, U.; Sugahara, K.
    rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha-1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate (2001), J. Biol. Chem., 276, 4834-4838.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
DV1, expression of soluble form of rib-2 protein in COS-1 cells Caenorhabditis elegans
expression of soluble form of EXT1 and EXT2 proteins in COS-1 cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
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 expressed fusion protein from COS-1after N-glycosidase treatment, SDS-PAGE Caenorhabditis elegans
130000
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 expressed fusion protein from COS-1, SDS-PAGE Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Homo sapiens elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
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 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Caenorhabditis elegans elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
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 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
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Homo sapiens
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-linked glycosylation, molecular weight of expressed fusion protein is reduced from 1300000 to 110000 by N-glycosidase treatment Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information EXT1 and EXT2 proteins also acts as beta(1,4)-glucuronyltransferase (EC 2.4.1.225) Homo sapiens ?
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 ?
additional information different acceptors tested for activity with UDP-N-acetyl-D-glucosamine Homo sapiens ?
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 ?
additional information different acceptors tested for activity with UDP-N-acetyl-D-glucosamine Caenorhabditis elegans ?
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 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
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 Homo sapiens UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
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 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
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 Caenorhabditis elegans UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
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 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Homo sapiens UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Caenorhabditis elegans UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
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 ?

Synonyms

Synonyms Comment Organism
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