Cloned (Comment) | Organism |
---|---|
expression of N-terminally His-tagged GSase in Escherichia coli strain BL21(DE3) or Top10 | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | over 90% inhibition | Streptomyces coelicolor | |
Cu2+ | over 90% inhibition | Streptomyces coelicolor | |
Ni2+ | over 90% inhibition | Streptomyces coelicolor | |
Zn2+ | over 90% inhibition | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1.5fold activation at 10 mM | Streptomyces coelicolor | |
Mn2+ | 10 mM, 47% of the activity with Mg2+ | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + [(1->4)-alpha-D-glucosyl]n | Streptomyces coelicolor | glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide | ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
ADP-glucose + [(1->4)-alpha-D-glucosyl]n | Streptomyces coelicolor A3(2) | glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide | ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged GSase from Escherichia coli strain BL21(DE3) or Top10 by nickel affinity chromatography and dialysis | Streptomyces coelicolor |
Storage Stability | Organism |
---|---|
-80°C, purified His-tagged enzyme, in 20 mM triethanolamine-HCl, pH 8.0, and 20% v/v glycerol, stable for at least 6 months | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + [(1->4)-alpha-D-glucosyl]n | glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide | Streptomyces coelicolor | ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
ADP-glucose + [(1->4)-alpha-D-glucosyl]n | substrate is rabbit liver glycogen | Streptomyces coelicolor | ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
ADP-glucose + [(1->4)-alpha-D-glucosyl]n | glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide | Streptomyces coelicolor A3(2) | ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
ADP-glucose + [(1->4)-alpha-D-glucosyl]n | substrate is rabbit liver glycogen | Streptomyces coelicolor A3(2) | ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE and gel filtration, quaternary structure determination, recombinant enzyme | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
GSase | - |
Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
metabolism | glycogen synthesis occurs via ADP-glucose in Streptomyces coelicolor, with the pathway being strictly regulated in connection with other routes involved with oligo- and polysaccharides, as well as with antibiotic synthesis in the bacterium, metabolic scenario for glucose 6-phosphate partitioning, overview | Streptomyces coelicolor |