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Literature summary for 2.4.1.21 extracted from

  • Senoura, T.; Asao, A.; Takashima, Y.; Isono, N.; Hamada, S.; Ito, H.; Matsui, H.
    Enzymatic characterization of starch synthase III from kidney bean (Phaseolus vulgaris L.) (2007), FEBS J., 274, 4550-4560.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
three recombinant proteins are constructed: putative mature recombinant SSIII (rPvSSII), recombinant kidney bean SSIII N-domain (rPvSSIII-N), and recombinant kidney bean SSIII C-domain (rPvSSIII-C) Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for amylopectin is 2.4 mg/ml, KM-value for glycogen is 8.7 mg/ml Phaseolus vulgaris
0.22
-
ADP-glucose pH 8.0, 30°C, at 10 mg/ml amylopectin Phaseolus vulgaris
0.32
-
ADP-glucose pH 8.0, 30°C,at 10 mg/ml glycogen Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris A4F2M4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Phaseolus vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + amylopectin N-domain of SSIII functions as a carbohydrate-binding module Phaseolus vulgaris ADP + ?
-
?
ADP-glucose + glycogen N-domain of SSIII functions as a carbohydrate-binding module Phaseolus vulgaris ADP + ?
-
?

Synonyms

Synonyms Comment Organism
starch synthase III
-
Phaseolus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable below Phaseolus vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
235
-
amylopectin pH 8.0, 30°C Phaseolus vulgaris
235
-
ADP-glucose pH 8.0, 30°C, at 10 mg/ml amylopectin Phaseolus vulgaris
333
-
ADP-glucose pH 8.0, 30°C, at 10 mg/ml glycogen Phaseolus vulgaris
412
-
glycogen pH 8.0, 30°C Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Phaseolus vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 10 stable Phaseolus vulgaris