Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
expression of full-length and truncated enzyme in Escherichia coli | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
additional information | - |
additional information | recombinant starch synthase I, amylopectin: 0.24 mg/ml, glycogen: 0.041 mg/ml | Zea mays | |
0.18 | - |
ADPglucose | starch synthase I, presence of 500 mM citrate | Zea mays | |
0.24 | - |
ADPglucose | starch synthase I, absence of citrate | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
starch grain | - |
Zea mays | 43036 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Zea mays | - |
starch synthase I | - |
Purification (Comment) | Organism |
---|---|
recombinant starch synthase I, ammonium sulfate, amylose column, Mono Q | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
unprimed reaction in the presence of 500 mM citrate, recombinant starch synthase I | Zea mays |
12.2 | - |
primed reaction, recombinant starch synthase I | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | recombinant starch synthase I in the presence of citrate | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10.3 | starch synthase II, sharp decline in activity below pH 7.0 | Zea mays |