Literature summary for 2.4.1.207 extracted from

Herbers, K.; Lorences, E.P.; Barrachina, C.; Sonnewald, U.
Functional characterisation of Nicotiana tabacum xyloglucan endotransglycosylase (NtXET-1): Generation of transgenic tobacco plants and changes in cell wall xyloglucan (2001), Planta, 212, 279-287.

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Cloned(Commentary)

Cloned (Comment)	Organism
NtXET-1 is cloned and expressed in Escherichia coli, ORF encodes a 295 amino acids protein, cDNA is cloned in sense and antisense orientation, creation of transgenic tobacco plants with reduced NtXET-1 expression: 2 independent lines with reduced total XET activity by 56% and 37%, respectively, in midribs of tobacco plants transformed with an antisense construct, which exhibit an at least 20% increase in the average MW of xyloglucan	Nicotiana tabacum

Cloned (Comment)

Organism

NtXET-1 is cloned and expressed in Escherichia coli, ORF encodes a 295 amino acids protein, cDNA is cloned in sense and antisense orientation, creation of transgenic tobacco plants with reduced NtXET-1 expression: 2 independent lines with reduced total XET activity by 56% and 37%, respectively, in midribs of tobacco plants transformed with an antisense construct, which exhibit an at least 20% increase in the average MW of xyloglucan

Nicotiana tabacum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	the same gene encodes a protein, that may be both soluble and bound to the cell wall	Nicotiana tabacum	5618	-
soluble	the same gene encodes a protein, that may be both soluble and bound to the cell wall	Nicotiana tabacum	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33900	-	x * 33900, calculated from the amino acid sequence	Nicotiana tabacum
36000	-	x * 36000, probably glycosylated enzyme, Western blot	Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
donor xyloglucan + xyloglucan acceptor	Nicotiana tabacum	NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Nicotiana tabacum	P93349	cv. Samsun NN, NtXET-1, belongs to group I of XET-related subfamilies	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	two putative glycosylation sites at amino acid positions 14 and 114	Nicotiana tabacum
proteolytic modification	contains a hydrophobic N-terminus indicating a signal peptide	Nicotiana tabacum

Purification (Commentary)

Purification (Comment)	Organism
recombinant NtXET-1, expressed in Escherichia coli	Nicotiana tabacum

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	source leaf, levels of NtXET-1 mRNA decreases in midribs with increasing age of leaves	Nicotiana tabacum	-
additional information	tissue-specific NtXET-1 expression pattern, highest mRNA levels in organs highly enriched in vascular tissues	Nicotiana tabacum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
donor xyloglucan + acceptor xyloglucan	NtXET-1 has a prefence for smaller xyloglucan molecules as acceptors	Nicotiana tabacum	?	-	?
donor xyloglucan + acceptor xyloglucan	donor: pine or tobacco xyloglucan	Nicotiana tabacum	?	-	?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol	pine or tobacco xyloglucan as donor	Nicotiana tabacum	?	-	?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol	XXXG-ol	Nicotiana tabacum	?	-	?
donor xyloglucan + xyloglucan acceptor	NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves	Nicotiana tabacum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 33900, calculated from the amino acid sequence	Nicotiana tabacum
?	x * 36000, probably glycosylated enzyme, Western blot	Nicotiana tabacum