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Literature summary for 2.4.1.207 extracted from

  • Steele, N.M.; Fry, S.C.
    Purification of xyloglucan endotransglycosylases (XETs): A generally applicable and simple method based on reversible formation of an enzyme-substrate complex (1999), Biochem. J., 340, 207-211.
No PubMed abstract available
Search for more literature for 2.4.1.207

General Stability

General Stability Organism
progressive purification destabilizes XET activity, 10% glycerol stabilizes Vigna radiata var. radiata
progressive purification destabilizes XET activity, 10% glycerol stabilizes Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, each isoenzyme, SDS-PAGE Vigna radiata var. radiata
32000
-
 x * 32000, each isoenzyme, SDS-PAGE Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 cauliflower
-
Brassica oleracea
-
 distinct isoforms
-
Vigna radiata var. radiata
-
 mung bean
-
Vigna radiata var. radiata
-
 distinct isoforms
-

Purification (Commentary)

Purification (Comment) Organism
general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms Vigna radiata var. radiata
general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
 Brassica oleracea
-
seedling
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.216
-
-
 Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Brassica oleracea ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Brassica oleracea ?
-
 ?
donor xyloglucan + acceptor xyloglucan XET forms a stable donor xyloglucan-XET complex Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + acceptor xyloglucan XET forms a stable donor xyloglucan-XET complex Brassica oleracea ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol donor: tamarind xyloglucan Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol donor: tamarind xyloglucan Brassica oleracea ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan Brassica oleracea ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol XLLG-ol Vigna radiata var. radiata ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol XLLG-ol Brassica oleracea ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, each isoenzyme, SDS-PAGE Vigna radiata var. radiata
? x * 32000, each isoenzyme, SDS-PAGE Brassica oleracea