Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.207 extracted from

  • de Silva, J.; Jarman, C.D.; Arrowsmith, D.A.; Stronach, M.S.; Chengappa, S.; Sidebottom, C.; Reid, J.S.G.
    Molecular characterization of a xyloglucan-specific endo-(1-->4)-beta-D-glucanase (xyloglucan endo-transglycosylase) from nasturtium seeds (1993), Plant J., 3, 701-711.
    View publication on PubMed
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
single copy gene, cDNA encoding XET is cloned, sequenced and encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein, NXG1 and 2 may be different alleles of the XET gene Tropaeolum majus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall localized exclusively in Tropaeolum majus 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SDS-PAGE and calculated from the amino acid sequence, the cDNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein Tropaeolum majus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
donor xyloglucan + xyloglucan acceptor Tropaeolum majus enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tropaeolum majus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Tropaeolum majus
proteolytic modification hydrophobic core of 24 amino acids at the N-terminus, characteristic of a signal peptide, enzyme must be transported through the cell membrane in order to reach the cell wall, DNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein Tropaeolum majus

Purification (Commentary)

Purification (Comment) Organism
-
 Tropaeolum majus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon source for mRNA isolation Tropaeolum majus
-
seed
-
 Tropaeolum majus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor xyloglucan specific for xyloglucan Tropaeolum majus ?
-
 ?
donor xyloglucan + xyloglucan acceptor enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression Tropaeolum majus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE and calculated from the amino acid sequence, the cDNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein Tropaeolum majus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.0-5.14 Tropaeolum majus