Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ruminococcus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | inhibition of the synthetic reaction, binds competitively at the glucose 1-phosphate site and uncompetitvely at the ternary enzyme-glucose 1-phosphate-glucose complex | Ruminococcus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.46 | - |
D-glucose | pH 6.0, 37°C | Ruminococcus albus | |
9.5 | - |
1,5-anhydro-D-glucitol | pH 6.0, 37°C | Ruminococcus albus | |
9.8 | - |
6-deoxy-D-glucose | pH 6.0, 37°C | Ruminococcus albus | |
10.8 | - |
D-gentobiose | pH 6.0, 37°C | Ruminococcus albus | |
13.3 | - |
D-glucosamine | pH 6.0, 37°C | Ruminococcus albus | |
23.5 | - |
D-mannose | pH 6.0, 37°C | Ruminococcus albus | |
25.5 | - |
D-xylose | pH 6.0, 37°C | Ruminococcus albus | |
60.2 | - |
2-deoxy-D-glucose | pH 6.0, 37°C | Ruminococcus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
2 * 94000, SDS-PAGE | Ruminococcus albus |
187000 | - |
gel filtration | Ruminococcus albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | - |
- |
- |
Ruminococcus albus NE1 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose | reaction proceeds through a random-ordered bi-bi mechanism, by which phosphate and cellobiose bind in random order and D-glucose is released before glucose 1-phosphate | Ruminococcus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol | - |
Ruminococcus albus | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol | - |
Ruminococcus albus NE1 | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Ruminococcus albus | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Ruminococcus albus NE1 | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Ruminococcus albus | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Ruminococcus albus NE1 | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-gentobiose | - |
Ruminococcus albus | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-glucosamine | - |
Ruminococcus albus | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-glucose | in synthesis reaction, glucose is best substrate | Ruminococcus albus | cellobiose + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-glucose | in synthesis reaction, glucose is best substrate | Ruminococcus albus NE1 | cellobiose + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-mannose | - |
Ruminococcus albus | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-xylose | - |
Ruminococcus albus | ? + phosphate | - |
r | |
cellobiose + phosphate | - |
Ruminococcus albus | alpha-D-glucose 1-phosphate + D-glucose | - |
r | |
cellobiose + phosphate | - |
Ruminococcus albus NE1 | alpha-D-glucose 1-phosphate + D-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 94000, SDS-PAGE | Ruminococcus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Ruminococcus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Ruminococcus albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
1,5-anhydro-D-glucitol | pH 6.0, 37°C | Ruminococcus albus | |
2.83 | - |
D-mannose | pH 6.0, 37°C | Ruminococcus albus | |
4 | - |
D-gentobiose | pH 6.0, 37°C | Ruminococcus albus | |
9.56 | - |
D-glucosamine | pH 6.0, 37°C | Ruminococcus albus | |
16.5 | - |
D-xylose | pH 6.0, 37°C | Ruminococcus albus | |
21.5 | - |
2-deoxy-D-glucose | pH 6.0, 37°C | Ruminococcus albus | |
92.3 | - |
D-glucose | pH 6.0, 37°C | Ruminococcus albus | |
124 | - |
6-deoxy-D-glucose | pH 6.0, 37°C | Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Ruminococcus albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.8 | stable within | Ruminococcus albus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
D-mannose | pH 6.0, 37°C | Ruminococcus albus | |
0.29 | - |
1,5-anhydro-D-glucitol | pH 6.0, 37°C | Ruminococcus albus | |
0.36 | - |
2-deoxy-D-glucose | pH 6.0, 37°C | Ruminococcus albus | |
0.37 | - |
D-gentobiose | pH 6.0, 37°C | Ruminococcus albus | |
0.65 | - |
D-xylose | pH 6.0, 37°C | Ruminococcus albus | |
0.72 | - |
D-glucosamine | pH 6.0, 37°C | Ruminococcus albus | |
12.7 | - |
6-deoxy-D-glucose | pH 6.0, 37°C | Ruminococcus albus | |
63.2 | - |
D-glucose | pH 6.0, 37°C | Ruminococcus albus |