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Literature summary for 2.4.1.20 extracted from
- Synthesis of cellobiose from starch by the successive actions of two phosphorylases (2010), N. Biotechnol., 26, 137-142.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzymatic cellobiose synthesis from starch using two phosphorylases: glucan phosphorylase, from Klebsiella pneumoniae, converts glucose residues in the starch into glucose-1-phosphate, removal of phosphate, and glucose-1-phosphate is incubated with alpha-D-glucose and is converted into cellobiose by recombinant cellobiose phosphorylase, removal of phosphate, 60% cellobiose yield from glucose 1-phosphate and, at least, 23.7% cellobiose yield from starch, method optimization, overview | Cellulomonas gilvus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + D-glucose | Cellulomonas gilvus | - |
cellobiose + phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas gilvus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + D-glucose | - |
Cellulomonas gilvus | cellobiose + phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
assay at | Cellulomonas gilvus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cellulomonas gilvus |
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Release 2023.1 (January 2023)
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