Crystallization (Comment) | Organism |
---|---|
at 25°C using the sitting-drop vapour diffusion method. Cellobiose phosphorylase-SO4 and cellobiose phosphorylase-PO4 structures are determined at 2.0 and 2.1 A resolution to R factor (Rfree) of 17.6% (21.3%) and 18.0% (22%) respectively. The pH values for crystallization are pH 8.2 for cellobiose phosphorylase-PO4 and pH 6.5 for cellobiose phosphorylase-SO4. | Cellulomonas gilvus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | 9 | 1,5-anhydroglucitol | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus | |
71 | - |
isomaltose | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus | |
83 | - |
gentiobiose | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus | |
93 | - |
melibiose | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas gilvus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cellulomonas gilvus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydroglucitol + glucose-1-phosphate | - |
Cellulomonas gilvus | ? | - |
? | |
gentiobiose + alpha-D-glucose 1-phosphate | - |
Cellulomonas gilvus | ? | - |
? | |
isomaltose + alpha-D-glucose 1-phosphate | - |
Cellulomonas gilvus | ? | - |
? | |
melibiose + alpha-D-glucose 1-phosphate | - |
Cellulomonas gilvus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Cellulomonas gilvus |
Synonyms | Comment | Organism |
---|---|---|
Cbp | - |
Cellulomonas gilvus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
isomaltose | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus | |
2.3 | - |
melibiose | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus | |
3.5 | - |
1,5-anhydroglucitol | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus | |
7.2 | - |
gentiobiose | enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) | Cellulomonas gilvus |