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Literature summary for 2.4.1.20 extracted from

  • Nidetzky, B.; Griessler, R.; Schwarz, A.; Splechtna, B.
    Cellobiose phosphorylase from Cellulomonas uda: gene cloning and expression in Escherichia coli, and application of the recombinant enzyme in a 'glycosynthase-type' reaction (2004), J. Mol. Catal. B, 29, 241-248.
No PubMed abstract available
Search for more literature for 2.4.1.20

Application

Application Comment Organism
synthesis ability of the phosphorylase to utilize alpha-D -glucose 1-fluoride as alternate glucosyl donor in place of alpha-D-glucose 1-phosphate for the synthesis of alpha-1,4-glucosides under thermodynamic control in close to 100% yield Cellulomonas uda

Cloned(Commentary)

Cloned (Comment) Organism
expression of a functional recombinant enzyme containing an N-terminal metal affinity fusion peptide in Escherichia coli. Catalytic properties of the natural enzyme are retained completely in the recombinant cellobiose phosphorylase Cellulomonas uda

Inhibitors

Inhibitors Comment Organism Structure
D-glucose substrate inhibition Cellulomonas uda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.82
-
 phosphate pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda
1.2
-
 phosphate pH 6.6, 30°C, native enzyme Cellulomonas uda
2.15
-
 alpha-D-glucose 1-phosphate pH 6.6, 30°C, native enzyme Cellulomonas uda
2.26
-
 D-glucose pH 6.6, 30°C, native enzyme Cellulomonas uda
2.9
-
 cellobiose pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda
3.28
-
 alpha-D-glucose 1-phosphate pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda
3.79
-
 D-glucose pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda
4.3
-
 cellobiose pH 6.6, 30°C, native enzyme Cellulomonas uda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Cellulomonas uda
92000
-
 1 * 92000, SDS-PAGE Cellulomonas uda

Organism

Organism UniProt Comment Textmining
Cellulomonas uda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Cellulomonas uda

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cellulomonas uda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D -glucose 1-fluoride + D-glucose
-
 Cellulomonas uda cellobiose + fluoride
-
 ir
alpha-D-glucose 1-phosphate + D-glucose
-
 Cellulomonas uda cellobiose + phosphate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 92000, SDS-PAGE Cellulomonas uda

Synonyms

Synonyms Comment Organism
CuCbP
-
 Cellulomonas uda

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.4
-
 phosphate pH 6.6, 30°C, native enzyme Cellulomonas uda
15.4
-
 phosphate pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda
27.5
-
 D-glucose pH 6.6, 30°C, native enzyme Cellulomonas uda
27.5
-
 D-glucose pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda
29.1
-
 cellobiose pH 6.6, 30°C, native enzyme Cellulomonas uda
29.1
-
 cellobiose pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda
43.2
-
 alpha-D-glucose 1-phosphate pH 6.6, 30°C, native enzyme Cellulomonas uda
43.2
-
 alpha-D-glucose 1-phosphate pH 6.6, 30°C, recombinant His6-tagged enzyme Cellulomonas uda

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
202
-
 D-glucose pH 6.6, 30°C, substrate inhibition, native enzyme Cellulomonas uda
255
-
 D-glucose pH 6.6, 30°C, substrate inhibition, recombinant His6-tagged enzyme Cellulomonas uda