Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | substrate inhibition, competitive with glucose-1-phosphate | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
D-glucose | - |
Acetivibrio thermocellus | |
5.9 | - |
D-glucosamine | - |
Acetivibrio thermocellus | |
6.1 | - |
6-deoxy-D-glucose | - |
Acetivibrio thermocellus | |
21.4 | - |
D-xylose | - |
Acetivibrio thermocellus | |
56.9 | - |
2-deoxy-D-glucose | - |
Acetivibrio thermocellus | |
131 | - |
D-mannose | - |
Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
SDS-PAGE, native PAGE | Acetivibrio thermocellus |
93760 | - |
calculated from DNA-sequence | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + phosphate | Acetivibrio thermocellus | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellobiose + phosphate | Acetivibrio thermocellus YM4 | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus YM4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from E. coli | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Acetivibrio thermocellus YM4 | ? | - |
? | |
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Acetivibrio thermocellus YM4 | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-glucosamine | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-mannose | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-xylose | no activity with L-isomer | Acetivibrio thermocellus | ? | - |
? | |
cellobiose + phosphate | - |
Acetivibrio thermocellus | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellobiose + phosphate | ordered bi-bi mechanism | Acetivibrio thermocellus | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellobiose + phosphate | beta-cellobiose preferred over alpha-anomer | Acetivibrio thermocellus | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellobiose + phosphate | - |
Acetivibrio thermocellus YM4 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellobiose + phosphate | ordered bi-bi mechanism | Acetivibrio thermocellus YM4 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellobiose + phosphate | beta-cellobiose preferred over alpha-anomer | Acetivibrio thermocellus YM4 | alpha-D-glucose 1-phosphate + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 92000, SDS-PAGE, native PAGE | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
D-glucosamine | - |
Acetivibrio thermocellus | |
9.3 | - |
D-mannose | - |
Acetivibrio thermocellus | |
12.2 | - |
D-glucose | - |
Acetivibrio thermocellus | |
18.3 | - |
2-deoxy-D-glucose | - |
Acetivibrio thermocellus | |
44.3 | - |
D-xylose | - |
Acetivibrio thermocellus | |
59.5 | - |
6-deoxy-D-glucose | - |
Acetivibrio thermocellus |